山香圆叶药材中鞣花酸的提取分离与含量测定  

Separaction and Dertermination of Ellagic Acid from Turpinia arguta

作　　者：杨小玲 刘地发 刘伟[3] 谢宁 程帆 刘鹏 欧阳婷 吕武清[4] 

机构地区：[1]江西青峰药业有限公司,江西赣州341000 [2]江西山香药业有限公司,江西赣州341000 [3]中国药科大学,南京210009 [4]江西省中医药研究院,南昌330046

出　　处：《中国实验方剂学杂志》2014年第10期54-57,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十二五"重大新药创制科技重大专项(2011ZX09102-009-03)

摘　　要：目的：建立山香圆叶药材中鞣花酸的制备工艺及含量测定方法，比较不同产地山香圆叶药材中鞣花酸的含量。方法：采用溶剂提取、大孔树脂及反相硅胶柱色谱和重结晶等提取分离手段得到鞣花酸对照品；用高效液相色谱法测定山香圆叶药材中鞣花酸的含量，Ultimate XB-C18色谱柱（4.6 mm250 mm，5 μm），流动相乙腈-0.1%磷酸（19∶81），流速1.0 mL·min^-1，检测波长254 nm，柱温30 ℃。结果：所得鞣花酸的纯度在98.5%以上，鞣花酸进样量在0.020 0-0.400 8 μg线性关系良好（r=0.999 8），平均回收率为101.25%；10个产地的山香圆叶中鞣花酸含量在0.37%-1.56%。结论：提取分离工艺得到的鞣花酸纯度高，可作为含量测定用对照品；含量测定方法简便、快速、定量准确，可用于山香圆叶中鞣花酸的含量测定；不同产地山香圆叶药材中鞣花酸的含量差异明显，为山香圆叶药材的资源开发提供了参考依据。Objective： To establish the methods for extraction,isolation and determ ination of ellagic acid from Turpinia arguta, and to compare its contents from different areas. Method： The ellagic acid reference substance was isolated by solvent extraction, macroporous adsorption resin, reverse phase silica gel column chromatog ramphy and recrystallization techniques. Then determined it by the method of HPLC from T. arguta, the chromatographic column was UltimateXB-C18 column（4.6 mm250 mm,5 μm）,the mobile phase was acetonitrile-0.1% phosphoric acid, the flow rate was at 1.0 mL·min^-1, the detection wavelength was 254 nm and the column temperature was 30 ℃. Result： The purity of ellagic acid obtained was higher than 98.5%,and the average recovery was 101.25%.The good linearity was achieved in the range of 0.020 0-0.400 8 μg. Ellagic acid content of T. arguta from 10 different areas was between 0.37% and 1.56%. Conclusion： Ellagic acid obtained from the method was with high purity, and it can be adopted as the reference substance. This method was simple,rapid and accuracy, it can be used to determine the contents of ellagic acid from T. arguta .The contents of ellagic acid were different in T. arguta from different areas. The result provided a reference for development of T. arguta resource.

关 键 词：山香圆叶 鞣花酸 提取分离 含量测定 

分 类 号：R284.1[医药卫生—中药学]