黄芪甲苷通过抑制Th2型细胞因子减轻变应性接触性皮炎  

Astragaioside IV Inhibits Allergic Contact Dermatitis through Reduction of Type 2 Cytokines Production

作　　者：魏筱[1,2] 洪敏[1,2] 沈丹丹[1,2] 桂黎黎[1,2] 江小燕[2,3] 于曦[1,2] 刘海亮[1,2] 江国荣[4] 

机构地区：[1]南京中医药大学药学院,南京210023 [2]国家规范化中药药理实验室江苏省中药药效与安全性评价重点实验室,江苏南京210029 [3]中国药科大学中药学院,南京210009 [4]苏州市中医医院,江苏苏州215003

出　　处：《中国实验方剂学杂志》2014年第10期121-124,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073121,81373549);江苏高校优势学科建设工程(YSXK-2010);“青蓝工程”项目;国家教育部留学回国人员科研启动基金

摘　　要：目的:应用异硫氰酸荧光素(FITC)诱导的Th2型小鼠变应性接触性皮炎的模型,评价黄芪甲苷(AS-IV)对Th2过敏性皮炎的作用。方法:BALB/c小鼠在day 1,day 2用1.5%的FITC溶液致敏腹部皮肤2次,同时给予地塞米松ip剂量为0.67 mg·kg-1,AS-IV(6.25,12.5,25,50 mg·kg-1,ig)至day 5(诱导相给药),day 6用0.5%FITC溶液攻击小鼠耳部皮肤,24 h后测定小鼠耳肿胀程度,耳组织HE染色,同时部分耳组织制匀浆以ELISA方法检测白介素(IL)-4,IL-13,IL-9水平。结果:与正常组比较,模型组小鼠耳肿胀明显,耳组织真皮层明显水肿,炎症细胞大量浸润,Th2型细胞因子IL-4,IL-13,IL-9水平显著升高;黄芪甲苷25,50 mg·kg-1诱导相给药能显著抑制模型的耳肿胀度;病理组织学检查亦显示与模型组比较,给予黄芪甲苷小鼠的耳炎症反应减轻,炎细胞浸润明显减少;同时能显著降低耳组织匀浆中IL-4,IL-13,IL-9水平。结论:黄芪甲苷诱导相给药对FITC诱导的小鼠Th2型变应性接触性皮炎有显著的抑制作用,该作用与抑制Th2型细胞因子的产生有关。Objective： Mice Type2 allergic contact dermatitis induced by fluoresciniso thiocyate （FITC） was utilized in this study to explore the effect of astragaioside IV （AS-IV） on allergic inflammatory. Method ： The BALB/c mice were topically sensitized on the shaved abdomens with 1.5% FITC solution at day 1 and day 2 and elicited on the right ear with 0.5% FITC at day 6. Mice in the dexamethasone group were intraperitoneal injection with dexamethasone （0.67 mg·kg^-1） and the AS-IV group were intragastrically administered with astragaioside IV （6.25, 12.5, 25, 50 mg ·kg^-1） from day 1 to day 5 （induction phase）. Auricle swelling was measured 24 h after the elicitation. Pathohistological examination was carried out by HE staining. Interleukin-4 （ IL-4）, IL-13 and IL-9 levels of ear tissue homogenates were detected by ELISA. Result： Compared with the normal group, ear swelling in model group was stimulated by FITC treatment significantly and ear tissue local edema, angiectasis and lymphocytic infiltration were observed simultaneously. Meanwhile, IL-4, IL-13 and IL-9 levels increased remarkably in FITC treated mice. Wherein, AS-IV 25 mg·kg^-1 and 50 mg·kg^-1 evidently inhibited the auricle inflammation of the FITC treated mice. Pathohistological results indicated that AS-IV alleviated local edema and angiectasis of mice models and reduced lymphocytic infiltration significantly. Furthermore, IL-4, IL-13 and IL-9 levels in ear tissue homogenates were markedly decreased by AS-IV treatment. Conclusion： Administration with AS-IV in induction phase could inhibit Th2 cell-mediated allergic contact dermatitis in mice significantly and this effect was related with regulation on type 2 cytokines.

关 键 词：黄芪甲苷 变应性接触性皮炎 白介素-4 白介素一13 白介素-9 

分 类 号：R285.5[医药卫生—中药学]