走马胎中三萜皂苷成分H1对6株肿瘤细胞增殖及对A549肺癌细胞凋亡及细胞周期的影响  

Effect of Triterpenoid Saponins H1 from Ardisia gigantifolia on Proliferation of Six Different Tumor Cell Lines and Apoptosis and Cell Cycle of A549 Tumor

作　　者：谷永杰[1,2] 穆丽华[2] 董宪喆[2] 姚成东 刘屏[2] 

机构地区：[1]河北北方学院,河北张家口075000 [2]中国人民解放军总医院临床药理研究室,北京100853

出　　处：《中国实验方剂学杂志》2014年第10期130-133,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(31000153)

摘　　要：目的：研究紫金牛科紫金牛属植物走马胎Ardisia gigantifolia中的三萜皂苷成分H1对不同肿瘤细胞株增殖的影响，并检测H1对A549肺癌细胞凋亡及细胞周期的影响。方法：以细胞密度为3107/L细胞悬液每孔90 μL接种于96孔板中，培养24 h后，各组分别加入不同浓度（终浓度分别为0，1.25，2.5，5，10，20 μmo·L^-1）的H1药液分别作用于MCF-7，HepG2，HeLa，A549，Bel-7402，BGC-823肿瘤细胞株24，48，72 h，并设阴性对照组。采用3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐（MTT比色法）检测H1对肿瘤细胞株增殖的影响，筛选对H1敏感的细胞株，用荧光倒置生物显微镜观察细胞形态学变化、采用流式细胞仪检测细胞凋亡和细胞周期。结果：H1作用于MCF-7，HepG2，HeLa等6株肿瘤细胞24，48，72 h后的IC50为4.26-6.45 μmo·L^-1，其中A549细胞株对H1最为敏感。终浓度为0，1.25，2.5，5 μmo·L^-1的H1干预A549细胞24 h后，中、高剂量组的细胞凋亡形态明显，发生早期凋亡；总凋亡率分别为2.2%，7.0%，14.2%，56.4%，各剂量组与正常组比较均有统计学意义；细胞阻滞发生在G2期，使S期急剧增加。结论：H1对6种肿瘤细胞株有一定程度的抑制作用，对A549细胞增殖的抑制作用较显著，其诱导A549细胞的凋亡机制可能与对细胞周期阻滞有关。Objective： To study the effect of triterpenoid saponins（H1） from Ardisia gigantifolia on the proliferation of different tumor cell lines. We select the A549 lung cancer cell line as target cell and detect the influence of triterpenoid saponins H1 on cell apoptosis and cell cycle. Method： The three different concentration of H1 （1.25, 2.5, 5 μmo·L^-1, were applied to target cells for 24, 48, 72 hours respectively. The control group was not treated. The MTT [3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide] colorimetric method was used to detect the tumor cell line proliferation and select the cell lines sensitive to H1.The fluorescent inverted biological microscope was used to observe cell morphology and cell apoptosis and cell cycle were detected by flow cytometry instrument. Result： After comparing the IC50 of H1 acted on the target cell line（MCF-7, HepG2, HeLa, A549, Bel-7402 and BGC-823） for 24, 48,72 h, the A549 cell line was proved to be the most sensitive one, a certain concentration of H1 intervention on A549 cells can lead to typical morphological changes and early apoptosis. Cell block was occurred in G2 period, which makes S period increased dramatically. The difference between Medium and high dose group and the negative control group differences were statistically significant. Conclusion： The H1 in a certain range of doses can inhibit the proliferation of six different tumor cell lines and select the A549 tumor cell as target on cell apoptosis and cell cycle.The mechanism of apoptosis induced by A549 cell may be related to cell cycle arrest.

关 键 词：走马胎 三萜皂苷 抗肿瘤活性 凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学]