叶绿酸铁对硝酸盐胁迫下黄瓜幼苗渗透调节物质及抗氧化酶活性的影响  

Effect of Chlorophyllin-iron on Osmoregulation Substance and Activities of Antioxidant Enzymes of Cucumber Seedling under Nitrate Stress

作　　者：张蒙[1] 王秀峰[1,2] 魏珉[1,2,3] 杨凤娟[1,2] 史庆华[1,2] 李岩[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]作物生物学国家重点实验室,山东泰安271018 [3]农业部黄淮海设施农业工程科学观测实验站,山东泰安271018

出　　处：《中国蔬菜》2014年第3期14-18,共5页China Vegetables

基　　金：国家现代农业产业技术体系建设专项(CARS-25)

摘　　要：采用营养液栽培,研究硝酸盐胁迫下喷施10mg·L-1叶绿酸铁溶液对黄瓜幼苗生长、脯氨酸、可溶性糖、丙二醛含量及抗氧化酶活性的影响。结果表明:硝酸盐胁迫下叶面喷施叶绿酸铁可以缓解硝酸盐对黄瓜幼苗生长的抑制,减少叶片中脯氨酸、可溶性糖、丙二醛含量,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)及抗坏血酸过氧化物酶(APX)活性。喷施叶绿酸铁可以在一定程度上减少硝酸盐胁迫下叶片中活性氧的积累,抑制膜脂过氧化,从而缓解硝酸盐胁迫对黄瓜幼苗的伤害。A solution culture experiment was conducted to study the effect of exogenous spraying 10 mg·L-1chlorophyllin-iron solution at cucumber（ Cucumis sativus L.）seedling leaves on the plant growth,contents ofproline,soluble sugar,MDA and activity of peroxidase under nitrate stress. The results showed that exogenousspraying chlorophyllin-iron solution at leaves could relieve the inhibition of nitrate on cucumber seedling growth,decrease the contents of proline,soluble sugar,MDA,and increase the activities of SOD,POD,CAT andAPX. Eexogenous spraying chlorophyllin-iron could to certain extent reduce the accumulation of active oxygenin leaves under nitrate stress, restrain lipid peroxidation,and therefore relieve the damage of nitrate stress oncucumber seedlings.

关 键 词：叶绿酸铁 硝酸盐胁迫 渗透调节物质 抗氧化酶 

分 类 号：S642.2[农业科学—蔬菜学]