南海红树林内生真菌ZH-3代谢产物研究  

Metabolites of Mangrove Endophytic Fungus ZH-3 from South China Sea

作　　者：杨建香[1,2] 邱声祥[1] 佘志刚[3] 林永成[3] 

机构地区：[1]中国科学院华南植物园植物资源保护与可持续利用重点实验室,广东广州510650 [2]桂林师范高等专科学校,广西桂林541001 [3]中山大学化学与化学工程学院,广东广州510275

出　　处：《化工技术与开发》2014年第2期1-3,共3页Technology & Development of Chemical Industry

基　　金：国家自然科学基金资助项目(81373293);国家863计划;支撑计划项目(201473948);广西教育厅科研项目(201204LX497)

摘　　要：研究南海红树林内生真菌ZH-3的代谢产物。采用反复硅胶柱色谱法、Sephadex LH-20凝胶色谱法等进行分离纯化,并通过理化常数测定和光谱分析鉴定其化学结构。从南海红树林内生真菌ZH-3的菌体中分离得到4个代谢产物,经波谱解析,分别为2-(3-chloro-2,6-dihydroxy-4-methylbenzoyl)-5-hydroxy-3-methoxybenzoate.(1),3'-dihydroxy-5,5'-dimethyldiphenyl ether(2),7,8-二甲基苯并[g]蝶啶-2,4(1H,3H)-二酮(3),5-o-methy lbostrycoidin(4)。初步药理活性显示化合物1对人体肝癌细胞hepG2抑制的IC50值为25μg·mL-1。所有化合物均首次从南海红树林内生真菌ZH-3中分离得到。Secondary metabolites of mangrove endophytic fungus No. ZH-3 from South China sea were studied. The compounds were isolated by column chromatography and identified on the basic of physic-chemical constants and spectral analysis. Four metabolites were obtained and elucidated as 2-（3-chloro-2,6-dihydroxy-4-methylbenzoyl）-5-hydroxy-3-methoxybenzoate. （1）,3,3’-dihydroxy-5,5’-dimethyldiphenylether（2）, 7,8-dimethylbenzo[g]pteridine-2,4（1H,3H）-dione （3）, 5-o-methylbostrycoidin （4）. In the preliminary bioassay, 1 show inhibittory activity on the hepG2 cell line with IC50values of 25μg/mL. The four metabolites were obtained from endophytic fungus No. ZH-3 for the ifrst time.

关 键 词：红树林 内生真菌 代谢产物 

分 类 号：O629[理学—有机化学]