不同种植方式下烤烟根系差异表达蛋白质分析  

Analysis of Root Differential Expression Proteins of Nicotiana Tabacum under Two Planting Systems

作　　者：尤垂淮[1,2] 陈冬梅[1,2] 黄锦文[1] 唐莉娜[3] 徐志兵[1,2] 张重义[1,2] 林文雄[1,2] 

机构地区：[1]福建农林大学农业生态研究所,福州350002 [2]福建农林大学烟草研究所,福州350002 [3]福建省烟草专卖局烟草农业科学研究所,福州350003

出　　处：《中国烟草科学》2014年第1期89-95,共7页Chinese Tobacco Science

基　　金：福建省烟草公司项目"福建烟区以烟为主耕作制度研究"{闽烟合同[2006]18号};"福建清香型烟叶适宜区生态基础研究"(闽烟合同[2010]-036)

摘　　要：通过根系差异蛋白质组学探讨了种植方式(复种连作、复种轮作)对烤烟生长的影响。结果表明,共有15个蛋白质表达丰度发生变化,用LC-MS/MS鉴定了14个差异蛋白质点,经生物信息学分析,11个蛋白质的功能有注释,其中3个属于与香气形成有关的蛋白质,包括转酮醇酶、单脱氢抗坏血还原酶和O-甲基转移酶,它们在复种轮作条件下均上调表达,此可能有助于烟叶品质提高,而复种连作处理下调表达,可能不利于品质提高。还鉴定到涉及能量、抗性、物质运输、核酸等功能的蛋白:磷酸甘油酸酯激酶、线粒体内膜移位酶亚基Tim13、NBS-LRR、DnaJ、细胞内囊泡运输蛋白Sly1、核糖体蛋白质,在复种轮作下也上调表达,此可能促进烤烟的生长,有利于提高烟草产量和品质且经济效益较好。而在复种连作处理下调表达,进而可能使得烟草生长变弱、产量降低、品质变差。Root differential proteomics was employed in this study to explore the impact of different planting patterns （multiplecropping rotation （MR） and consecutive monoculture multiple cropping （MC） modes） on growth of flue-cured tobacco （Nicotinatobaccum L）. The results showed that a total of 15 protein spots had significant expression difference; 14 protein spots wereidentified by LC-MS/MS analysis and database searching. Among which, the function of 11 proteins were identified, three proteinswere related to the aroma formation, i.e. transketolase, monodehydroascorbate reductase（MDHA）, O-methyltransferase-like proteinwere up-regulated under MR, it was helpful probably to enhance the tobacco quality. While under the MC treatment, they weredown-regulated, and the tobacco quality could be worse. Phosphoglycerate kinase, mitochondrial import inner membrane translocasesubunit Tim13, NBS-LRR（nucleotide binding site plus leucine-rich repeat）, DnaJ protein, Vesicle trafficking protein Sly1, Ribosomalprotein-like protein were involved in energy metabolism, plant antioxidation reaction, translocation of solute, metabolism of nucleicacid, respectively. They were very important for the growth development of tobacco and all up-regulated under MR treatments,probably increasing the yield and improving the quality of tobacco, and gaining better economic benefit. They were alldown-regulated under MC, which may cause weak growth, low yield and poor quality of tobacco.

关 键 词：烤烟 双向电泳 蛋白质组学 

分 类 号：TS41[农业科学—烟草工业]