SYSMEX XN-1000全自动血液分析仪计数有核红细胞的准确性评价  

The accuracy evaluation of nucleated red blood cell count determined by SYSMEX XN-1000 automatic hematology analyzer

作　　者：王也飞[1] 周怡[1] 丁磊[1] 夏文权[1] 胡翊群[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院检验系,上海200025

出　　处：《检验医学》2014年第3期262-265,共4页Laboratory Medicine

摘　　要：目的探讨SYSMEX XN-1000全自动血液分析仪(简称XN-1000)计数有核红细胞(NRBC)的准确性。方法应用XN-1000对66例外周血标本进行NRBC检测,同时用流式细胞仪(FCM)和显微镜(镜检法)对同一份外周血的NRBC百分率进行计数。以镜检法为金标准,比较不同方法的计数结果。结果 XN-1000与镜检法检测NRBC有较好的相关性(r=0.927),但低于FCM与镜检法NRBC的相关性(r=0.956),XN-1000与FCM检测NRBC的相关性较高(r=0.994)。XN-1000、FCM计数结果与镜检法的结果间差异均无统计学意义(P>0.05)。XN-1000检测NRBC%较FCM法有更高的诊断特异性(95.7%)。结论 XN-1000能快速、准确、有效地自动计数外周血NRBC。Objective To investigate the accuracy of nucleated red blood cell （NRBC）count determined by SYSMEX XN-1000 automatic hematology analyzer（XN-1000）.Methods A total of 66 peripheral blood samples were collected and determined for NRBC by XN-1000,and the results of NRBC% were compared with those by flow cytometry （FCM）and manual microscopy for the same sample.The manual microscopy was as golden standard.Results There was a good correlation for NRBC between XN-1000 and microscopy （r =0.927 ）,but the correlation was lower than that between FCMand manual microscopy （r=0.956）.The correlation between XN-1000 and FCMwas the best （r=0.994）. The results of XN-1000 and FCMwith manual microscopy had no statistical significance（P〉0.05）.XN-1000 for NRBC%had a better specificity （95.7%） than FCM.Conclusions XN-1000 automatic hematology analyzer has good performance for detecting NRBC in peripheral blood rapidly,accurately and effectively.

关 键 词：SYSMEX XN-1000全自动血液分析仪 有核红细胞 准确性 

分 类 号：R466.11[医药卫生—临床医学]