阿托伐他汀通过PPARβ/δ途径下调老年大鼠心肌细胞IL-1β表达  

Atorvastatin down-regulating IL-1β expression in myocyte of aging rat by PPARβ / δ signal channel

作　　者：韩磊[1] 叶平[2] 李鸣皋[1] 

机构地区：[1]中国人民解放军海军总医院全军航海航空医学中心,北京市100048 [2]中国人民解放军总医院老年心血管二科,北京市100853

出　　处：《实用医学杂志》2014年第9期1363-1366,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:70872713)

摘　　要：目的:观察阿托伐他汀下调老年大鼠心肌细胞白细胞介素-1β(interleukin-1β,IL-1β)表达的作用与过氧化物酶体增殖物激活型受体β/δ(peroxisome proliferator activated receptorβ/δ,PPARβ/δ)信号途径的关系。方法:分离培养24月龄大鼠心肌细胞,分为空白对照组、溶剂对照组、阿托伐他汀组、PPARβ/δ拮抗剂GSK0660+阿托伐他汀组。分别加入细胞培养液、二甲基亚砜(dimethyl sulfoxide,DMSO)、阿托伐他汀、阿托伐他汀+GSK0660处理。分别用RT-PCR和Western blot方法检测各组细胞IL-1βmRNA和蛋白含量。结果:(1)与空白对照组相比,溶剂对照组细胞的IL-1βmRNA和蛋白水平均无显著差异(P>0.05);(2)与空白对照组相比,阿托伐他汀组的IL-1βmRNA蛋白水平均显著降低(P<0.01);(3)阿托伐他汀+GSK0660组的IL-1βmRNA和蛋白水平均显著高于阿托伐他汀组(P<0.05或P<0.01),但仍低于空白对照组(P<0.05)。结论 :阿托伐他汀可激活PPARβ/δ信号通路来下调衰老心肌细胞IL-1β的表达。Objective To investigate the correlation between atorvastatin inhibiting Interleukin- 1β （IL- 1β） expression and peroxisome proliferator activated receptor β/δ （PPARβ/δ） signal channel in myocyte of aging rat. Methods Primary culture of myocyte were got from aging rat. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GSK0660 group, whichwere treated respectively by Cell culture medium, dimethyl sulfoxide （DMSO）, atorvastatin, atorvastin plus GSK0660. The expression level of IL-1β mRNA and protein was evaluated by RT-PCR and Western Blot respectively. Results （1）NO difference were found between control group and DMSO group in expression level of IL-1β mRNA and protein （P 〉 0.05） ; （2）The expression level of IL-1β mRNA and protein in atorvastatin group were significantly lower than those of control group （P 〈 0.01） ; （3）Both mRNA and protein expression level of IL-1β in atorvastatin plus GSK0660 group were higher than those of atorvastatin group（P 〈 0.05 or P 〈 0.01 ）, but still lower than those of control group（P 〈 0.05）. Conclusions Atorvastatin down- regulate the expression of IL-1β in aging myocytes by activating PPARβ/δ signal Channel.

关 键 词：衰老 阿托伐他汀 过氧化物酶体增殖物激活型受体β δ 白细胞介素-1Β 大鼠 心肌细胞 

分 类 号：R965[医药卫生—药理学]