胸骨上小切口大鼠主动脉瓣上缩窄模型的建立  

Construction and evaluation of rat supravalvular aortic stenosis model through small incision on the suprasternal fossa

作　　者：马杰[1] 余杨[2] 蹇召 唐富琴[1] 肖颖彬[1] 陈林[1] 

机构地区：[1]第三军医大学附属新桥医院全军心血管外科研究所,重庆400037 [2]重庆市中山医院

出　　处：《中华实验外科杂志》2014年第5期1147-1149,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81170216）

摘　　要：目的探讨非人工通气条件下经胸骨上小切口建立大鼠主动脉瓣上缩窄模型的可行性和有效性。方法将90只大鼠随机分为主动脉瓣上缩窄组（n=50）和假手术组（n=40），在自主呼吸条件下，经胸骨上窝剪开胸骨至第2肋间，沿气管向下分离出升主动脉并结扎；假手术组仅行开胸处理，记录术前及术后1、3、6、9、12周的心脏超声、组织病理学检查结果，以独立样本t检验等进行比较分析。结果缩窄组大鼠生存率为84．0％，假手术组为92．5％。超声心动图检查显示：术后3周，缩窄组大鼠左心室呈明显向心性肥厚，舒张期左室后壁厚度为[（2．20±0．24）mm]、左心室重量指数为[（3．11±0．69）mg／g]与假手术组比较差异有统计学意义（P〈0．05）；术后9周，缩窄组大鼠左室呈现离心性肥厚，左室收缩末内径为[（3．25±0．61）mm]、舒张末内径为[（5．64±0．97）mm]显著增加（P〈0．05）；术后12周，缩窄组大鼠出现失代偿性心力衰竭的症状，左心室射血分数为[（41．00±9．23）％]显著降低（P〈0．05）。组织学检查显示：心肌细胞排列紊乱，细胞核增大，胞质增多，间质胶原纤维增生明显。结论非人工通气下经胸骨上小切口能成功建立大鼠升主动脉缩窄模型，且该模型具有操作简单、创伤小、重复性好的特点。Objective To investigate rat supravalvular aortic stenosis model via small incision on the suprasternal fossa without artificial ventilation. Methods Sprague-Dawley rats were randomly assigned to the aortic banding group （n = 50） and the sham-operation group （n =40）. The former underwent aortic banding through small incision on the suprasteInal fossa without artificial ventilation, and the latter received the same procedures but without actual ligation of the aorta. Echocardiography and myocardial pathological examination were implemented to observe how the heart physiological parameters changed before and after operation. The results were analyzed by using independent sample t test. Results The survival rate was 84% in banding group, while 92. 5% in sham group. Echocardiography examination showed： （1） At 3rd week after surgical operation, the left ventricle in banding group, which was different from sham-operation group, exhibited obvious concentric hypertrophy. Significant difference in the data on left ventricular posterior wall thickness [LVPW, （2. 20 ±0. 24） mm] and left ventricular mass index [LVMI, （3.11 ±0. 69） mg/g] was observed. The above indicators showed a slight upward trend at 6th, 9th, and 12th week after surgical operation; （2） At 9th week after surgical operation, the left ventricle in banding group got eccentric hypertrophy and the values of left ventricular endsystolic dimension [ LVESd, （3.25 ±0. 61 ） mm] and left ventrieular end-diastolic diameter [ LVEDd, （ 5.64 ± 0. 97 ） mm] increased markedly; （ 3 ） At 12th week later, the symptoms of decompensated heart iailure appeared in banding group. The left ventricular ejection fractions [ （41. 00 ± 9. 23 ）% ] decreased dramatically. Histological examinations revealed that the banding group exhibited disorganized cardiomyocytes, enlarged nuclei and increased cytoplasmic, and significant intersti- tial collagen fibers. However, the sham group displayed normal structure. Conclusion These facts confirm tha

关 键 词：小切口 非人工通气 升主动脉缩窄 左室肥厚 模型 动物 

分 类 号：R654.2[医药卫生—外科学]