姜黄素对胰岛素抵抗3T3-L1前脂肪细胞糖脂代谢及细胞分化的影响  

Influences of curcumin on carbohydrate metabolism and differentiation of insulin-resistant 3T3-L1 preadipocytes

作　　者：秦培洁[1] 张东伟[1] 莫芳芳[1] 赵丹丹[1] 李小可[1] 方心[1] 穆倩倩 于娜[1] 高思华[1] 

机构地区：[1]北京中医药大学,北京10029

出　　处：《北京中医药大学学报》2014年第4期236-239,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81274041);国家国际科技合作资助项目(No.2011DFA30920)

摘　　要：目的 探讨姜黄素对前脂肪细胞葡萄糖摄取、分化的影响及其机制.方法 用高浓度葡萄糖、胰岛素联合诱导培养的方法,建立脂肪细胞胰岛素抵抗模型并检测姜黄素、干预的脂肪细胞对3H-葡萄糖的摄取,对姜黄素干预分化的细胞进行油红O染色并通过比色定量分析脂肪细胞分化程度.实时定量荧光PCR检测脂肪细胞分化相关基因过氧化物体增殖剂活化受体γ（PPARγ）和CAAT/增强子结合蛋白A（C/EBPα） mRNA的表达.结果 姜黄素组葡萄糖摄取率为正常组的134.51％;姜黄明显促进脂肪细胞分化及PPARγ和C/EBPαmRNA其表达,与对照组比较,差异有统计学差异（P＜0.05）.结论 姜黄素能够促进脂肪细胞葡萄糖摄取及细胞分化,PPARγ和C/EBPαmRNA表达.Objective To discuss the influences of curcumin on carbohydrate metabolism and differentiation of preadipocytes and mechnism. Methods The model of adipocyte insulin-resistant was established by using culturing-inducing combination method of high-dose glucose and insulin. The uptake of 3H-glucose was detected in adipocytes after inventioned with curcumin, and the differentiation degree of adipocytes was analyzed by using spectrophotography quantitatively after oil red O staining. The expressions of PPARγ/ and C/EBPα were detected by using real-time fluorescence quantitative polymerase chain reaction （RT-PCR）. Results The uptake rate of glucose was 134.51% in curcumin group compared with control group, and the differentiation of adipocytes and expressions of PPARγ/mRNA and C/EBPα mRNA were improved significantly compared with control group （P 〈 0.05）. Conclusion Curcumin can improve glucose uptake and differentiation of adipocytes and expressions of PPARγ/mRNA and C/EBPa mRNA.

关 键 词：脂肪细胞 姜黄素 糖代谢 分化 

分 类 号：R285.5[医药卫生—中药学]