富油微藻——尖状栅藻生物质生产与奶牛场废水处理相结合的效果研究  

Integrated the biomass production of oleaginous microalga Scenedesmus acuminatus and dairy wastewater treatment

作　　者：高保燕 沈丹丹[1] 何思思[1] 万凌琳[1] 刘天中[2] 李爱芬[1] 张成武[1] 

机构地区：[1]暨南大学水生生物研究中心生态学系,广东广州510632 [2]中国科学院生物燃料重点实验室中国科学院青岛生物能源与过程研究所,山东青岛266101

出　　处：《可再生能源》2014年第5期673-679,共7页Renewable Energy Resources

基　　金："863"主题项目(2013AA065805);自然科学基金(31170337);广东省低碳专项(2011-051);中国科学院生物燃料重点实验室开放基金(CASKLB201302)

摘　　要：尖状栅藻（Scenedesmus acuminatus）是一株新近分离的富含油脂淡水绿藻，分别选用4种不同NaNO3初始浓度的BG－11培养基和4种不同浓度的奶牛场废水，在φ3．0 cm柱状光生物反应器中对其进行培养。试验结果显示，在BG-11培养纰中，NaNO，初始浓度为6．0mmol／L时尖状栅藻的生物量最大，达到9．5 g／L；3．6mmol／L时总脂含量最高，为藻体干重的62．6％。在废水培养组中，100％废水培养时藻生物量最大，达到12．2g/L；25％的废水培养时总脂含量最高，为藻体干重的62．4％；尖状栅藻的脂肪酸碳链长度为14～18 C，与石化柴油的平均碳链长度十分相近；在培养过程中该藻能有效地去除废水中的氮和磷．去除效率分别达到93．2％和99．4％。利用奶牛场废水培养富含油脂的尖状栅藻（S．acuminatus）不仅能够有效去除废水中的氮和磷等营养成分，还能为生物柴油生产提供有价格竞争优势的原料。Scenedesmus acuminatus was a new isolated freshwater green microalga which couldaccumulate high content of storage lipids. It was cultivated in four different initial NaNO3 con-centrations （18.0, 9.0, 6.0 mmol/L and 3.6 mmol/L） of BG-11 media and four different diluted con-centrations （25%, 50%, 75% and 100%） of dairy wastewater with φ3.0 cm column photobioreactors,respectively. The maximum biomass concentration reached 9.5 g/L in BG-11 medium containing6.0 mmol/L NaNO3, and the highest total lipid content was up to 62.6% of dry biomass in BG-11medium containing 3.6 mmol/L NaNO3. The maximum biomass concentration of 12.2 g/L was ob-tained in the 75% wastewater. The highest lipid content was gained in the 25% dilution ofwastewater, and the total lipid content reached 62.4% of dry biomass. The carbon chain length offatty acids in S. acuminatus was between C14 and C18 which was very similar to the averagecarbon chain length of the petroleum diesel. S. acuminatus could remove nitrogen and phosphoruseffectively from the difterent diluted dairy wastewater, and the removal efficiency reached 93.2%（N） and 99.4% （P）. S. acuminatus cultivated in the dairy wastewater was not only removed nitrogenand phosphorus effectively, but also could be a price competitive producer of biodiesel.

关 键 词：尖状栅藻 奶牛场废水 油脂 单位体积产率 生物柴油 

分 类 号：TK6[动力工程及工程热物理—生物能] S216.2[农业科学—农业机械化工程]