单扫描示波极谱法测定血中铅方法建立  

Determination of lead in blood by single scan Oscillographic Polarography

作　　者：邱倩[1] 

机构地区：[1]赣榆县疾病预防控制中心,江苏222100

出　　处：《医学动物防制》2014年第4期463-465,共3页Journal of Medical Pest Control

摘　　要：目的采用三电极系统测定铅(Ⅱ)离子的峰电流,建立单扫描示波极谱法测定血中铅的方法。方法在盐酸-碘化钾-酒石酸钾钠-抗坏血酸混合体系中,采用单扫描示波极谱法测铅(Ⅱ)的极谱峰。结果峰电位为-540mv,铅离子的线性范围为0.01μg/ml^0.3μg/ml,线性回归方程为Y=52.93x+12.88,相关系数为0.9 998,最低检出限为0.005μg/ml,采用本法测定血中铅(Ⅱ),样品回收率在95.0%~104.8%之间,相对标准偏差RSD在1.03%~2.82%之间。结论本法准确、简便、快速,结果满意。Objective By using three electrode system （ II ） to determinate the peak current of lead ion, to build a method of determination of Pb （ II ） by single scan Oscillographic Polarography. Methods In the solution of HC1- KI- C4H406KNa -C6HsO10, adopting a single scan Oscillographic Polarography lead （ II ） polar graphic peak. Results Wave peak was observed at -540my, the peak current was proportional to the concentration of Pb （ II ） in range of 0. 01 -0.3μg/ml, the linear regression equation was Y = 52.93x ＋ 12.88, the related coefficient was 0. 9998, the lowest detection limit was 0. 005μg/ml, the method was applied to determination of Pb （ II ） in the sample , the recovery rate was 95.0% - 104. 8%, the RSD obtained was 1.03% -2. 82%. Conclusions It is concluded that the method has the advantage of high accura- cy, simplicity, quickness and satisfactory results.

关 键 词：血中铅 单扫描示波极谱法 

分 类 号：R446.112[医药卫生—诊断学]