基于纳米银/石墨烯/酪氨酸酶/聚酰胺-胺的双酚A传感器  

Bisphenol A biosensor based on glassy carbon electrode modified with Ag nanoparticles, graphene, tyrosinase and polyamidoamine

作　　者：郭倩琼 王炜祺[1] 高佩怡[1] 马腾飞[1] 杨乐乐[1] 黄杉生[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《化学传感器》2014年第1期23-29,共7页Chemical Sensors

摘　　要：采用石墨烯纳米材料,并结合酪氨酸酶、聚酰胺-胺(PAMAM)和纳米银修饰玻碳电极研制了新型BPA生物传感器。运用循环伏安法和电化学交流阻抗考察了修饰电极的电化学行为。由于石墨烯独特的物理化学性质,结合聚酰胺-胺和纳米银的协同作用,该修饰电极对于BPA有较好的电流响应。在最佳条件下,该传感器对双酚A的线性检测范围为1.0×10-7～3.3×10-5mol/L,检测限为3.0×10-8 mol/L(信噪比为3),相关系数为0.998。A bisphenol A biosensor based on Ag nanoparticles, graphene, tyrosinase and polyamidoamine （PAMAM） modified electrode was prepared. The electrochemical behavior of the BPA biosensor was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. Owning to the special physicochemical characteristic of the nanomaterials and the synergism between Ag nanoparticles and PAMAM, the sensor showed good amperometric response towards BPA. Under the optimal experimental conditions, the current is linear with the concentration of BPA in the range from 1.0 ×10^-7 to 3.3×10^-5 mol/L, with a detection limit of 3.0×10^-8 mol/L （S/N = 3） and a correlation coefficient of 0.998.

关 键 词：石墨烯 酪氨酸酶 聚酰胺-胺 纳米银 双酚A传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]