Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury  

作　　者：Zhiyuan Li Zhanxiu Zhang Lili Zhao Hui Li Suxia Wang Yong Shen 

机构地区：[1]Department of Joint Orthopedics,Hebei Provincial Xingtai People’s Hospital [2]Department of Spinal Orthopedics,Third Hospital of Hebei Medical University

出　　处：《Neural Regeneration Research》2014年第8期806-814,共9页中国神经再生研究（英文版）

摘　　要：We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells before transplantation might further improve neurological function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone marrow mesenchymal stem cell group, and significantly greater compared with the model group. After 4 weeks, behavioral performance was signiifcantly enhanced in the model group. Af-ter 8 weeks, the number of horseradish peroxidase-labeled nerve ifbers was higher in the Nogo-66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and signiifcantly higher than in the model group. The newly formed nerve ifbers and myelinated ner ve ifbers were detectable in the central transverse plane section in the bone marrow mesenchymal stem cell group and in the Nogo-66 receptor gene silencing group.We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells before transplantation might further improve neurological function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone marrow mesenchymal stem cell group, and significantly greater compared with the model group. After 4 weeks, behavioral performance was signiifcantly enhanced in the model group. Af-ter 8 weeks, the number of horseradish peroxidase-labeled nerve ifbers was higher in the Nogo-66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and signiifcantly higher than in the model group. The newly formed nerve ifbers and myelinated ner ve ifbers were detectable in the central transverse plane section in the bone marrow mesenchymal stem cell group and in the Nogo-66 receptor gene silencing group.

关 键 词：nerve regeneration spinal cord injury bone marrow mesenchymal stem cells Nogo-66receptor RNA interference horseradish peroxidase BRDU gene silencing neural regeneration 

分 类 号：R651.2[医药卫生—外科学]