Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation  

作　　者：Wang Li Bing Wang Man Wang Min Chen Jing-Ming Yin Ghullam Murtaza Kaleri Rui-Jie Zhang Tie-Niu Zuo Xiong You Qing Yang 

机构地区：[1]College of Life Sciences, Nanjing Agricultural University

出　　处：《Journal of Integrative Plant Biology》2014年第4期364-372,共9页植物学报（英文版）

基　　金：supported financially by grants from the Jiangsu Agricultural Science and Technology Innovation Fund (cx (11)1020);National Natural Science Foundation of China (11171155);A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions: Modern horticultural science (PAPD)

摘　　要：Anthocyanins are a class of products of plant secondary metabolism and are responsible for tubers color in potato. The biosynthesis of anthocyanins is a complex biological process, in which multiple genes are involved including structural genes and regulatory genes. In this study, StAN11, a WD4o-repeat gene, was cloned from potato cultivar Chieftain （Solanum tuberosum L.）. StAN11 （HQ5995o6） contained no intron and its open reading frame （ORF） was 1,o29 bp long, encoding a putative protein of 342 amino acids. In order to verify its role in anthocyanin biosynthesis, StAN11 was inserted behind the CaMV-35S promoter of pCMBIA1304 and the recombination vector was introduced into the potato cultivar Desiree plants by Agrobacterium-mediated transfor- mation. The color of transgenic tuber skin was significantly deepened, compared to the wild-type control, which was highly consistent with the accumulation of anthocyanin and expression of StAN11 in transgenic lines tuber skin. Further analysis on the expression of Flavonone-3-hydroxylase （F3H）,Dihydroflavonol reductase （DFR）, Anthocyanidin synthase （ANS）, and F1avonoid 3-O-glucosyl transferase （3GT） in transgenic plants revealed that only DFR was upregulated. This result suggested that StAN11 regulated anthocyanin biosynthesis in potato by controlling DFR expression and accumulation of anthocyanin could be increased through overexpression of .StAN11 in the tubers with the genetic background of anthocyanin biosynthesis.Anthocyanins are a class of products of plant secondary metabolism and are responsible for tubers color in potato. The biosynthesis of anthocyanins is a complex biological process, in which multiple genes are involved including structural genes and regulatory genes. In this study, StAN11, a WD4o-repeat gene, was cloned from potato cultivar Chieftain （Solanum tuberosum L.）. StAN11 （HQ5995o6） contained no intron and its open reading frame （ORF） was 1,o29 bp long, encoding a putative protein of 342 amino acids. In order to verify its role in anthocyanin biosynthesis, StAN11 was inserted behind the CaMV-35S promoter of pCMBIA1304 and the recombination vector was introduced into the potato cultivar Desiree plants by Agrobacterium-mediated transfor- mation. The color of transgenic tuber skin was significantly deepened, compared to the wild-type control, which was highly consistent with the accumulation of anthocyanin and expression of StAN11 in transgenic lines tuber skin. Further analysis on the expression of Flavonone-3-hydroxylase （F3H）,Dihydroflavonol reductase （DFR）, Anthocyanidin synthase （ANS）, and F1avonoid 3-O-glucosyl transferase （3GT） in transgenic plants revealed that only DFR was upregulated. This result suggested that StAN11 regulated anthocyanin biosynthesis in potato by controlling DFR expression and accumulation of anthocyanin could be increased through overexpression of .StAN11 in the tubers with the genetic background of anthocyanin biosynthesis.

关 键 词：Anthocyanin biosynthesis cloning genetic transformation POTATO StAN11 

分 类 号：S532[农业科学—作物学]