直接器官发生途径的榉树微繁体系建立  

Micro Propagation System Establishment of Zelkova Schneideriana Through a Direct Organogenesis Way

作　　者：刘雪梅[1] 罗雪梅[1] 王征[1] 金晓玲[1] 

机构地区：[1]中南林业科技大学风景园林学院,中国长沙410004

出　　处：《湖南师范大学自然科学学报》2014年第2期17-21,共5页Journal of Natural Science of Hunan Normal University

基　　金：湖南省科技计划资助项目(2011NK3040);湖南省研究生科研创新基金资助项目(CX2012B329);中南林业科技大学研究生科技创新基金资助项目(CX2012B06)

摘　　要：为了建立通过直接器官发生途径的榉树微繁技术体系，以当年生带芽茎段为外植体，进行了榉树不定芽的直接诱导试验．结果表明：适合不定芽诱导的培养基为MS＋BA2．25—4．50μmol／L，有效芽诱导率达到76．67％；继代增殖培养的培养基为MS＋BA2．25μmol／L，增殖系数为5．1；壮苗培养基为MS＋BA2．25μmol／L＋GA31．45μmol／L；生根培养基为1／2MS＋NAA2．69μmol／L＋活性炭0．1g·L^-1，生根率77．78％，生根数达4．5条．In order to establish Zelkova schneideriana regeneration system through a direct organogenesis way, the current-year stems with a bud was used as explants. Results show that it was cultured on Murashige and Skoog＇s （MS） medium that supplemented with 2.25 μmol/L to 4.50 μmol/L 6-benzylaminopurine （BA） to induce adventitious bud, and 76.67% of stems with a bud can form adventitious bud. Then the adventitious bud was subcul- tured on MS containing 2.25 μmol/L BA to propagate shoots, and approximately five shoots per-explants were propagation. Shoots were cuhured on MS supplemented with 2.25 ixmol/L BA and 1.45 μmol/L Gibberellin A3 （ GA3 ） could be got sound seedling. The shoots were rooted on 1/2MS containing 2.69 μmol/L naphthylene acetic acid （NAA） and 0.1 g · L^-1 activated carbon, on which 77.78% shoots developed roots with average of 4.5 roots per-shoot.

关 键 词：榉树 不定芽 直接器官发生 再生体系 

分 类 号：Q943.1[生物学—植物学]