肝脾调补方对大鼠急性酒精性肝损伤的防护作用研究  

Experimental study of Ganpitiaobu decoction on alcoholic induced liver injury in rats

作　　者：阮连国[1] 张妍[2] 吴建红[3] 朱清静[3] 张赤志[4] 

机构地区：[1]湖北中医药大学 [2]武汉市妇女儿童医疗保健中心 [3]武汉市医疗救治中心 [4]湖北省中医院

出　　处：《中西医结合肝病杂志》2014年第2期104-105,108,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：武汉市卫生局资助项目(No.WX08D19)

摘　　要：目的:观察中药肝脾调补方对大鼠急性酒精性肝损伤的防护作用。方法:采用白酒灌胃法建立大鼠急性酒精性肝损伤模型。通过肝脾调补方高、中、低剂量灌胃,观察肝脾调补方对各组大鼠血清ALT、AST、肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)及肝组织匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮(NO)水平的影响。结果:肝脾调补方可明显降低大鼠血清ALT、AST、TNF-α、IL-6水平;降低肝组织匀浆的NO的含量,提高肝匀浆SOD、GSH-Px活性。结论:肝脾调补方对急性酒精性大鼠肝损伤有防护作用,能改善肝功能,其机制可能与减轻肝脏炎症、抗脂质过氧化有关。Objective： To study the effects of Ganpitiaobu decoction in protecting alcoholic injury in mice and study on its pharmacological mechanism. Methods： Acute hepatic injury model was made by alcohol administration by gavage. Sixty mice were divided into controlgroup, modelgroup, low dose group, middle dose group and high dose group randomly. The levels of an inotransferase （ALT）, aspartate an inotransferase （AST）, in blood plasma and liver homogenateofsuperoxidedismutase （SOD）, glutathione peroxidase （GSH-PX） , nitric oxide （NO）, TNF-α, IL-6 of the five groups were examined. Results： Ganpitiaobu decoction could significantly reduce ALT, AST, TNF-α, IL-6 content in mice serum and NO in liver hamogenate, elevat liver homogenate contents of SOD, GSH-PX. Conclusion ： Ganpitiaobu decoction can obviously protect alcoholic injury to liver in mice by reducing ere inflammation of the liver and anti lioid oeroxidation

关 键 词：酒精性肝损伤 肝脾调补方 药效学 作用机制 大鼠 

分 类 号：R285.5[医药卫生—中药学]