提高生物指示剂菌片指示菌回收率的方法研究  

RESEARCH ON THE METHOD FOR IMPROVING THE SPORE RECOVERY RATE OF BIO-INDICATOR STRIPS

作　　者：朱以萍[1] 谢清[1] 黄力勤[1] 张瑞丰[1] 陆兵[2] 吕京[3] 

机构地区：[1]中国科学院微生物研究所,北京100101 [2]军事医学科学院生物工程研究所 [3]中国合格评定国家认可中心

出　　处：《中国消毒学杂志》2014年第4期346-348,共3页Chinese Journal of Disinfection

基　　金："十二五"国家科技支撑计划(2011 BAI 15 B03);卫生公益性行业科研专项(201302006)

摘　　要：目的研究生物指示剂菌片指示菌回收方法,以提高指示菌回收效率。方法采用菌片破碎法,再以常规活菌培养计数方法对指示菌进行培养计数。结果采用菌片破碎法可使进口生物指示剂菌片指示菌平均回收率达83.2%,使国产生物指示剂菌片指示菌平均回收率达70.2%;相比之下,用传统的振荡法指示菌的平均回收率均≤1.0%。结论采用菌片破碎法,生物指示剂菌片指示菌回收效率明显优于传统的振荡法。Objective To develop a spore extracting method for efficiently improving the spore recovery rate when doing spore enumeration of bio - indicator strips. Methods The BI strips smash method was used to extract spores from the strips before performing spore enumeration. Results With this method, the mean recovery rate was 83.2% for SGM BI strips and 70. 2% for Sihuan BI strips, while it was ≤ 1.0% for SGM BI strips by vortex method. Conclusion The estab- lished strip smash method is much more efficient than the conventional vortex method.

关 键 词：生物指示剂 菌片 破碎法 振荡法 回收率 

分 类 号：R181.2[医药卫生—流行病学]