急性心肌缺血再灌注大鼠早期心脏舒张功能降低及心肌纤维化的研究  

Cardiac CVF and HYP and serum level of TNF-αin rats with acute MI/R

作　　者：邵靓[1] 张萍[2] 张勇[1] 马爱群[1] 

机构地区：[1]西安交通大学医学院第一附属医院心内科,西安710061 [2]湖北省武汉市华中科技大学附属协和医院神经内科

出　　处：《陕西医学杂志》2014年第5期518-521,525,共5页Shaanxi Medical Journal

基　　金：国家自然科学基金青年科学基金项目(No 81300115);陕西省自然科学基金(No 2013JM4041)

摘　　要：目的:探讨急性心肌缺血再灌注大鼠心功能的改变及心肌纤维化对其影响。方法:建立大鼠急性心肌缺血再灌注模型,利用心脏超声和血流动力学检测其心功能状态。用水解法检测心肌羟脯氨酸含量。分别用免疫印迹和酶联免疫吸附剂测定法检测心肌和血清中TNF-α的表达。结果:急性心肌缺血再灌注大鼠早期出现心脏舒张功能降低,在灌注第3天时尤为显著。心肌纤维化发生在缺血再灌注区,且该部位胶原容积分数显著增高,与心肌TNF-α表达规律相同。同时心肌羟脯氨酸含量和血清TNF-α在急性缺血再灌注时也显著增加。结论:急性心肌缺血再灌注大鼠心肌纤维化降低早期心脏舒张功能,而炎症因子TNF-α的异常增高是急性心肌缺血再灌注心肌纤维化的可能因素之一。Objective：To discuss the cardiac function changes and the myocardial fibrosis effects in acute myocardial ischemia/reperfusion rats .Methods ：Acute myocardial ischemia/reperfusion rats models were estab-lished for detecting their heart function by echocardiography and hemodynamic .Water solution method was used to detect expression of myocardial hydroxyproline .And the expression of TNF-αin the myocardial and serum was de-tected by western blot and enzyme-linked immunosorbent method .Results ：Early heart diastolic function was lower in acute myocardial ischemia/reperfusion rat ,significantly in the third day after perfusion .Myocardial fibrosis oc-curred in ischemia/reperfusion areas ,and the collagen volume fraction was significantly increased in this area .It was identical to expression of TNF-αin myocardial .Meanwhile ,myocardial hydroxyproline content and TNF-αin serum also significantly increased in acute ischemia/reperfusion .Conclusion：Myocardial fibrosis results to deterioration of early heart diastolic functionin in rats with acute myocardial ischemia/reperfusion .And the expression of inflamma-tory cytokine TNF-αis one of the possible factors of myocardial fibrosis in acute myocardial ischemia/reperfusion .

关 键 词：心肌缺血 病理生理学 心肌再灌注损伤 病理生理学 羟脯氨酸 代谢 肿瘤坏死 因子 代谢 酶联免疫吸附测定 模型 动物 大鼠 

分 类 号：R542.23[医药卫生—心血管疾病]