苯丙氨酸和茉莉酸甲酯对百里香离体培养芽生长及其精油的影响  

Study on the Effects of Phenylalanine and Methyl Jasmonate on Profile of Essential Oil from Tissue Culture of Thymus vulgaris L.

作　　者：李晓东[1] 徐世千[2] 鲁朝辉[1] 崔淑芬[1] 张建国[2] 

机构地区：[1]深圳职业技术学院化生学院,广东深圳518055 [2]华南农业大学农学院,广东广州510641

出　　处：《香料香精化妆品》2014年第2期1-6,共6页Flavour Fragrance Cosmetics

基　　金：国家自然科学基金(编号81072615);深圳市科技项目(编号JC200903180714A)

摘　　要：以离体培养的百里香不定芽为材料,研究了培养基添加不同浓度的苯丙氨酸(phenylalanine,Ple)或茉莉酸甲酯(methyl jasmonate,MJ)对不定芽生长以及精油提取率等的影响。结果发现添加供试浓度的Ple和MJ都能不同程度促进不定芽的增殖,其中以添加100 mg/L Ple和150μmol/L MJ增殖效果最佳,增殖系数分别比对照显著增加47.03%和23.57%。采用水蒸气蒸馏法提取了普通百里香(Thymus vulgaris L.)组培苗、以及培养基添加苯丙氨酸或茉莉酸甲酯的普通百里香[0]组培苗的精油,添加100 mg/L Ple和150μmol/L MJ的提取率分别为0.42%和0.39%,分别比对照(0.31%)增加了35.5%和25.8%。利用气质联用法(GC-MS)并结合色谱峰面积归一化法对其化学成分和相对含量进行了比较研究,3种组培苗百里香精油的主要化学成分无明显的差异,都含有百里香精油的主要成分百里香酚、香芹酚、邻-伞花烃、γ-松油烯、石竹烯等,只是相对含量略有差异。研究表明,适宜浓度的苯丙氨酸和茉莉酸甲酯不仅提高了百里香不定芽的增殖系数,改善了植株的生理活性,而且还促进了百里香次生代谢产物的积累和合成。The effects of phenylalanine（Ple） and methyl jasmonate（MJ） on growth of in vitro adventitious buds of Thymus vulgaris L.and profile of their essential oils were studied.The results showed that by adding different concentrations of Ple and MJ,the proliferation of adventitious buds could be promoted,and the best proliferation was found when 100 mg/L Ple and 150 μmol/L MJ were added,compared with the control,the proliferation coefficients were significantly increased 47.03 ％ and 23.57％ respectively.The essential oils from the tissue culture seedlings,and the tissue culture seedlings treated with phenylalanine（Ple） and methyl jasmonate（MJ）were extracted by steam distillation,the chemical constituents and their relative contents of these three samples were identified and analyzed by gas chromatography-mass spectrometry with peak area normalization method.The result showed that the main constituents of these three samples had no significant difference,they all mainly contained thymol,carvacrol,o-cymene,γ-terpinene,caryophyllene,etc.,only slight differences in the relative contents were observed.This meant suitable concentration of Ple and MJ could increase proliferation coefficient of adventitious buds of Thymus vulgaris L.and improve the physiological activities of the plant,also,the accumulation and synthesis of secondary metabolites within this palnt were promoted.

关 键 词：离体培养 百里香 苯丙氨酸 茉莉酸甲酯 精油 

分 类 号：TQ651[化学工程—精细化工]