南海海洋沉积物中抗肿瘤活性菌株的初步筛选与鉴定  

Screening and identification of the marine bacteria with antitumor activity from marine sediment of the South China Sea

作　　者：夏玉林[1] 程力慧[1] 李荷[1] 

机构地区：[1]广东药学院基础学院,广东广州510006

出　　处：《广东药学院学报》2014年第1期76-80,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省科技厅项目(2012B01030002)

摘　　要：目的从南海海洋沉积物中分离筛选出具有抗肿瘤活性的菌株,并对其进行鉴定。方法利用MTT法对筛选菌株粗提取物进行抗肿瘤活性筛选,并对具有明显细胞毒性的菌株进行16S rDNA系统发育学分析。结果在分离得到的11株海洋细菌中,菌株S24具有明显的细胞毒性。16S rDNA系统发育学分析表明,菌株S24与Pectobacterium cypripedii果胶杆菌属的16S rDNA序列相似性为99%。结论海洋沉积物来源的细菌是抗肿瘤活性物质的重要来源,是开发抗肿瘤药物的宝贵资源。Objective To screen and identify the marine bacteria with antitumor activity from marine sediment of the South China Sea. Methods MTF assay was used to screen the antitumor activity from crude extracts of the marine bacterial strains, and the phylogenetic analysis of the 16S rDNA sequence was examined in the strain with obvious cytotoxicity. Results The strain S24 showed an obvious cytotxic activity among the 11 tested bacterial isolates. The phylogenetic analysis of the 16S rDNA sequence showed that the similarity of the 16S rDNA sequence between the strain S24 and Pectobacterium cypripedii was 99%. Conclusion The bacteria from marine sediment is an important source of anti-tumor activity substances, and it is also a precious resource of developing anti-tumor medicaments.

关 键 词：海洋沉积物 海洋细菌 抗肿瘤活性 16S RDNA 

分 类 号：R979.1[医药卫生—药品]