体外分离培养的心脏干细胞具有间充质干细胞的特性  

Mouse Adult Cardiac Resident Stem Cells Exhibit Mesenchymal Features

作　　者：王丽娜[1,2] 宋祥和 刘艳华[2] 苏位君[2] 周曼倩[2,4] 童玲玲[2] 王宝玉[4] 郑国光[1] 李宗金[2] 

机构地区：[1]中国医学科学院血液学研究所实验血液学国家重点实验室,天津300020 [2]南开大学医学院,天津300071 [3]山东省日照市中医院,山东日照276800 [4]南开大学人民医院,天津300121

出　　处：《南开大学学报（自然科学版）》2014年第2期46-51,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(81371620;81300376);协和青年基金和中央高校基本科研业务费专项基金(3332013067)

摘　　要：近年来,已报道从成年的小鼠中分离得到心脏干细胞(Cardiac Stem.Cells CSCs),并且应用于心脏病的治疗.但是心脏干细胞特征尚未完全阐述.通过体外分离成年小鼠的心肌组织,进行培养,分离得到半悬浮状态的具有干细胞特性的细胞;利用流式细胞仪检测分离得到的心脏干细胞的表面分子表达情况;并且通过调整培养条件诱导其体外分化.研究结果显示成功分离得到的心脏干细胞,表达干细胞标志分子Sca-1,以及间充质干细胞标志分子(CD29、CD90、CD44和CD106);体外分化研究显示心脏干细胞可以分化成心肌细胞、平滑肌细胞、脂肪细胞和成骨细胞.心脏干细胞具有间充质干细胞的特性,具有多向分化潜能,在心脏损伤的治疗中具有重要意义.Cardiac Stem Ceils （CSCs） have been isolated from heart of adult mouse and hu- man, and have shown beneficial effects in the therapy of ischemia heart disease. However, the characters of the CSCs were not fully elucidated. In this study, myocardial tissues from adult mouse heart were minced, digested and cultured. The phase-bright cells were harvested after subculture. In vitro characterization was done by fluorescence-activated cell sorting （FACS） analysis. In vitro differentiation of CSCs was carried out. The results demonstrated CSCs were obtained successfully; FACS analysis data revealed that this population cells express resident CSC markers （Sca-1） and exhibit mesenchymal features with expression of mesenchymal stem cell markers （CD29, CD90, CD44 and CD106）. Afterwards, these cells can differentiate into cardiomyocytes, smooth muscle ceils, adipocytes and osteoblasts. In conclusion, CSCs exhibit mesenchymal features and can successfully differentiate into different cell lineages.

关 键 词：心脏干细胞 心球样细胞团 间充质干细胞 分化 

分 类 号：Q26[生物学—细胞生物学]