Simultaneous quantification of eighteen constituents in traditional Chinese medicine XiaoShuanTongLuo by UPLCMS/ MS  

UPLC-MS/MS法同时测定消栓通络方中18种成分的含量(英文)

作　　者：赵艳[1,2] 刘敏[2] 杨钊 王少华[2] 李杨[2] 杜冠华[4] 

机构地区：[1]青岛大学医学院,山东青岛266071 [2]青岛大学医学院附属青岛市市立医院,山东青岛266011 [3]青岛市药检所,山东青岛266071 [4]中国医学科学院北京协和医学院药物研究所国家药物筛选中心,北京100050

出　　处：《Journal of Chinese Pharmaceutical Sciences》2014年第4期225-232,共8页中国药学（英文版）

基　　金：National Natural Science Foundation of China(Grant No.30630073)

摘　　要：XiaoShuanTongLuo （XSTL） is an important multi-herbal formula widely used as a traditional Chinese medicine （TCM）. In this paper, an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method （UPLC-ESI-MS/MS） was developed for the simultaneous identification and quantification of 18 chemical constituents in the extract of XSTL. The analysis was performed in multiple-reaction monitoring （MRM） mode using positive and negative electrospray ionization （ESI）. A BEH C18 （2.1 mm× 100 mm, 1.7 μm） column with a gradient mobile phase of methanol-0.1% aqueous formic acid solution at a flow rate of 0.2 mL/min was used for separation. The standard curves of most components showed good linearity over the concentration range of 0.001-0.5 μg/mL with r = 0.9896-0.9996. The relative standard deviations （RSD） of intra-day and inter-day precision were all lower than 15% at low, middle, and high concentration levels. The RSD of stability analysis in 24 h were also lower than 15%. The validated method was employed to quantify 18 active components simultaneously in four different commercial brands of XSTL products in various dosage forms. The developed method was rapid, accurate, sensitive, and reproducible for the quality control of XSTL.消栓通络方是临床应用广泛的中药复方制剂。

关 键 词：Traditional Chinese medicine XiaoShuanTongLuo Ultra-performance liquid chromatography Triple quadrupole tandem mass spectrometer Quality control 

分 类 号：R284.1[医药卫生—中药学]