血管内皮生长因子单核苷酸多态性与子痫前期易感性的相关研究  

Analysis on single nucleotide polymorphism of vascular endothelial growth factor and susceptibility of preeclampsia patients

作　　者：张竑卉 何云[1] 张彩媚[1] 

机构地区：[1]深圳市妇幼保健院产科,广东深圳518000

出　　处：《现代医学》2014年第4期353-357,共5页Modern Medical Journal

基　　金：深圳市科技研发资金知识创新计划基础研究项目(JCYJ20130402091224668)

摘　　要：目的:探讨血管内皮生长因子(VEGF)+936C/T、-634G/C两个位点单核苷酸多态性与子痫前期易感性的相关性。方法:采用双脱氧链末端终止法进行DNA测序,对2012年1月至2013年1月在本医院住院分娩的58例子痫前期,70例正常妊娠女性的VEGF+936C/T、-634G/C单核苷酸位点进行基因分析,比较各组基因型和等位基因频率分布有无差异。用优势比(OR)计算VEGF+936C/T基因在子痫前期患病中的相对危险度,比较两组中两个位点不同等位基因孕妇的血压、血肌酐、24 h尿蛋白定量等临床指标。结果:子痫前期组VEGF+936CT、TT基因型、T等位基因高于正常对照组,VEGF+936CC基因型、C等位基因低于正常对照组,差异有统计学意义(P<0.05);VEGF-634G/C位点的基因型、等位基因频率分布在子痫前期组及正常对照组孕妇中比较,差异均无统计学意义(P>0.05);正常对照组、子痫前期组人群中携带VEGF+936CT、TT基因型组发生子痫前期的危险性是CC基因型组的2.557倍;子痫前期组+936C/T位点CT+TT基因型孕妇与CC基因型孕妇的各临床指标比较,-634G/C位点GC+GG基因型孕妇与CC基因型孕妇各临床指标比较差异均有统计学意义(P<0.05);而对照组+936C/T位点CT+TT基因型孕妇与CC基因型孕妇的各临床指标比较,-634G/C位点GC+GG基因型孕妇与CC基因型孕妇各临床指标比较,差异均无统计学意义(P<0.05)。结论:携带VEGF+936CT、TT基因型者患子痫前期的危险性增加,-634G/C位点的G等位基因孕妇比携带C等位基因者更具有子痫前期遗传易感性,提示VEGF单核苷酸多态性可能与子痫前期的易感性相关。Objective： To investigate the potential association between ＋936C/T and-634G/C polymorphism of vascular endothelial growth factor（ VEGF） gene and susceptibility of preeclampsia. Methods： Fifty-eight preeclamptie patients and seventy normal term pregnant women（ as control group） were recruited from the Department of Obstetrics in our hospital during the period from January 2012 to January 2013. Genotype and allele frequencies of ＋ 936C / T,-634G / C were determined by the dideoxy-mediated chain termination method. The relative risk of ＋ 936 G / C gene in preeclampsia was calculated by the odds ratio（ OR）. Compared the clinical and biochemical parameters for different alleles between the preeclamptie patients and controls for statistical significance respectively,such as blood pressure,serum creatinine and 24-hour urineprotein. Results： VEGF ＋ 936 CT,TT genotype and T allele in the preeclamptic group was higher than that in the control group,VEGF ＋ 936CC and C allele genotype in the preeclamptic group was lower than that in the control group,and the difference was statistically significant（ P〈0. 05）. No significant differences were found in the genotype and allele frequencies of-634G / C polymorphisms between the two groups（ P〉0. 05）. The incidence of preeclampsia in the CT and TT genotype was 2. 557 times of CC genotype. In the preeclamptic group,some clinical and biochemical parameters were significantly different in women with the ＋ 936AG ＋ GG genotypes compared with AA genotype,the-634 GC ＋ GG genotypes compared with CC genotype（ P〈0. 05）. Conclusion： The cases with ＋ 936 CT,TT genotypes have high risk of preeclampsia. VEGFS-634 G allele carriers has more genetic predisposition than C allele carriers in preeclampsia. This study suggests that polymorphisms of VEGF gene might be associated with the susceptibility of preeclampsia.

关 键 词：子痫前期 血管内皮生长因子 单核苷酸多态性 易感性 

分 类 号：R714.245[医药卫生—妇产科学]