Screening of Drought-tolerant Brassica napus L.Varieties and Analysis on Their Physiologic and Biochemical Variations under Drought Stress  

耐旱性甘蓝型油菜品种的筛选及其在干旱胁迫下生理生化特征的研究（英文）

作　　者：李淑娟[1] 程量 彭少丹[2] 陈升位[1] 王勤方 林良斌[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学农科专业基础实验教学中心,云南昆明650201

出　　处：《Agricultural Science & Technology》2014年第4期596-604,615,共10页农业科学与技术（英文版）

基　　金：Supported by Rapeseed Industry Construction Program of Department of Agriculture of Yunnan Province;Fund for Workstation of Academician Guan Chunyun from Department of Science and Technology of Yunnan Province~~

摘　　要：[Objective] The aim of this study was to screen drought-tolerant Brassica napus L. germplasm resources by analyzing their physiological and biochemical changes under drought stress. [Method] Forty varieties of B. napus varieties were cultured under PEG-6000 osmotic stress and extreme drought stress in pots, re- spectively. Then, the contents of chlorophyll, carotenoid, proline, malondialdehyde （MDA）, soluble sugar, soluble protein, and the activities of superoxide dismutase （SOD）, catalase（CAT）, peroxidase（POD） were measured under drought stress. [Result] Sever- al drought-tolerant varieties of B. napus were screened out： YAU200908, Xiangyou No.15, YAU200903, YAU200907, YAU200906 and YAU200904. Physiological and biochemical analysis showed that, the contents of chlorophyll and carotenoid de- creased with drought stress increasing; the contents of proline, soluble sugar, solu- ble protein, MDA and the activities of SOD,CAT, POD raised with drought stress in- creasing. [Conclusion] In the rapeseed varieties with stronger drought tolerance, the decrease in carotenoid content and the increase in proline content, soluble sugar content, MDA content, SOD activity, CAT activity were more obvious, so all these physiological and biochemical indices can be used to evaluate the drought tolerance of rapeseed.[目的]筛选甘蓝型油菜耐旱种质资源,并分析其干旱胁迫下生理生化变化。[方法]以40个不同甘蓝型油菜品种(系)为材料,采用PEG-6000模拟生理干旱胁迫和盆栽极限干旱胁迫等方法进行耐旱种质资源的筛选,并对干旱胁迫下叶绿素含量、类胡萝卜素含量、脯氨酸含量、丙二醛(MDA)含量、可溶性糖含量、可溶性蛋白含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性的变化进行了研究。[结果]筛选鉴定出一批耐旱性甘蓝型油菜品种:YAU200908、湘油15号、YAU200903、YAU200907、YAU200906、YAU200904。生理生化分析表明,在干旱胁迫下,油菜叶片中叶绿素、类胡萝卜素的含量随干旱程度的加剧而降低,脯氨酸、可溶性糖、可溶性蛋白、MDA的含量及SOD、CAT、POD的酶活性则随着干旱程度的加剧而升高。[结论]在抗旱性强的材料中,其类胡萝卜素的含量显著地减少,脯氨酸、可溶性糖、MDA的含量及SOD、CAT的酶活性显著增加,可作为油菜抗旱性鉴定的生理生化指标。

关 键 词：Brassica napus L. Drought tolerance Drought stress Physiologic and biochemical changes 

分 类 号：S634.3[农业科学—蔬菜学]