山核桃叶总黄酮诱导人多发性骨髓瘤U266细胞株凋亡作用研究  

Apoptotic effects of total flavonoid from the leaves of Carya cathayensis Sarg. on U266

作　　者：翟斡[1] 沈嫣婧[2] 沈一平[1] 丁志山[2] 

机构地区：[1]浙江省中医院,浙江杭州310006 [2]浙江中医药大学,浙江杭州310053

出　　处：《甘肃中医学院学报》2014年第2期7-11,共5页Journal of Gansu College of Traditional Chinese Medicine

摘　　要：目的研究山核桃叶总黄酮诱导人多发性骨髓瘤(U266)细胞株凋亡的作用及其作用机制。方法体外培养U266细胞,CCK-8比色法检测细胞增殖活性,流式细胞术定量分析细胞凋亡程度,Western Blot分析不同浓度总黄酮对U266细胞蛋白激酶B(Akt)和p-Akt蛋白表达的影响。结果山核桃叶总黄酮以浓度依赖性方式显著诱导U266细胞株凋亡并抑制p-Akt蛋白的表达。结论山核桃叶总黄酮诱导U266细胞凋亡的作用与其抑制Akt磷酸化相关。Objective To observe the apoptotic effects of total flavonoids from the leaves of Carya cathayensis Sarg. on U266 and their underlying mechanisms. Methods U266 were cultured in vitro, cell proliferation was measured by CCK-8. Cell apoptosis was assayed by flow cytometry. The expression of Akt and p-Akt of U266 after treatment with different concentrations of total flavonoids was analyzed by western blot. Results Total flavonoids from the leaves of Carya cathayensis Sarg. significantly induced U266 apoptosis and inhibited the expression of p-Akt in a concentration-dependent manner. Conclusion The apoptotie effects of total flavonoids from the leaves of Carya cathayensis Sarg. on U266 was associated with the inhibition of phosphorylation of Akt.

关 键 词：人多发性骨髓瘤 U266细胞株 山核桃叶总黄酮 凋亡 蛋白激酶B P-AKT 

分 类 号：R285.5[医药卫生—中药学]