肝素对劳力性热射病大鼠模型早期干预的作用  

Role of heparin in early treatment of exertional heat stroke in rats

作　　者：陈洁坤 宋青[1] 李瑞生[2] 张婷[1] 苗利辉[1] 吴振华[1] 

机构地区：[1]解放军总医院重症医学科,北京100853 [2]解放军第302医院动物实验中心,北京100039

出　　处：《解放军医学院学报》2014年第5期470-473,共4页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(81050005)~~

摘　　要：目的探讨肝素对劳力性热射病(exertional heat stroke,EHS)大鼠模型早期干预的作用。方法雄性SD大鼠60只随机分为3组:空白对照组(A组,n=8)、劳力性热射病组(B组,n=26)、劳力性热射病+肝素组(C组,n=26)。B组及C组应用热应激+运动建立劳力性热射病大鼠模型。成模后B组、C组随机取10只做生存率分析(B1组、C1组),B组和C组其余大鼠按发病后0 h、2 h随机分为两个亚组(B2组、B3组、C2组、C3组,各8只)。取血测定血小板(platelet,PLT)、部分活化凝血活酶时间(activated partial thromboplastin time,APTT),应用ELISA方法测定肿瘤坏死因子-α(TNF-α)含量。结果 C1组8 h生存率高于B1组(P<0.05)。与A组相比,B2组、B3组、C2组、C3组PLT均显著降低(P<0.01),APTT显著延长(P<0.01)。但与同时间点B组相比,C2组、C3组血小板数量升高(P<0.01),APTT短于B组(P<0.01)。与A组相比,B2组、B3组、C3组TNF-α显著升高(P<0.01);但C2组、C3组TNF-α明显低于同时间点B组(P<0.01)。结论肝素预处理可提高EHS模型大鼠生存率,改善其凝血功能异常及降低炎症反应,在一定程度上缓解EHS的进展。Objective To study the role of heparin in early treatment of exertional heat stroke （EHS） in rats. Methods Sixty male SD rats were randomly divided into blank control group （group A, n=8）, exertional heat stroke group （group B, n=26）, and exertional heat stroke ＋ heparin group （group C, n=26）. A rat EHS ＋ exercise model was established, then the survival rate of 10 rats randomly selected from group B and group C （group B1 and group C1） was analyzed. The remaining rats in groups B and C were randomly divided into group B2, B3 and group C2, C3 （8 in each group）. Their platelets （PLT） and activated partial thromboplastin time （APTT） were calculated and their serum tumor necrosis factor- α （TNF- α ） level was measured by ELISA. Results The 8 h survival rate was significantly higher in group C1 than in group B1 and significantly lower in group B2, B3 and group C2, C3 than in group A （P 〈 0.05, P 〈 0.01）. The APTT was significantly longer in group B2, B3 and group C2, C3 than in group A （P 〈 0.01） and significantly shorter in group C than in group B （P 〈 0.01）. The number of PLT was significantly higher in group C2, C3 than in group B （P 〈 0.01）. The serum TNF- α level was significantly higher in group B2, B3, C2 and group C3 than in group A and significantly lower in group C2, C3 than in group B （P 〈 0.01）. Conclusion Heparin pretreatment can increase the survival rate of EHS rats by improving their coagulation function, reducing their inflammatory reactions and inhibiting their EHS progression.

关 键 词：热射病 肝素 凝血 炎症 

分 类 号：R873[医药卫生—运动医学]