贝伐珠单抗引起肝癌细胞HepG2的血管内皮生长因子受体2上调表达  

Bevacizumab up-regulates expression of VEGFR2 in HepG2 cells

作　　者：刘洪金[1] 赵忠鹏[2] 付艳[1] 杨鹏辉[2] 段跃强[2] 门丽[2] 赵晖[1] 杜楠[1] 王希良[2] 

机构地区：[1]解放军总医院第一附属医院肿瘤二科,北京100048 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《解放军医学院学报》2014年第5期474-476,488,共4页Academic Journal of Chinese PLA Medical School

基　　金：北京科委首都医疗特色项目(2131107002213040)~~

摘　　要：目的探讨贝伐珠单抗对肝癌细胞HepG2血管内皮生长因子(vascular endothelial growth factor,VEGF)相关受体的影响。方法通过不同浓度的贝伐珠单抗降低培养液中VEGF来模拟低浓度VEGF的肿瘤微环境,RT-PCR、ELISA、Western blotting检测肿瘤细胞VEGF相关受体表达水平的变化。结果细胞培养液的VEGF浓度大幅度下降(P<0.05);可溶性VEGFR2的浓度出现了上升(P<0.05);HepG2细胞VEGF和VEGFR1表达水平无明显变化(P>0.05);VEGFR2表达水平出现了明显的上调(P<0.05)。结论贝伐珠单抗导致了肝癌细胞HepG2大幅度上调了VEGFR2的表达。Objective To study the role of bevacizumab in up-regulation of VEGFR2 expression in HepG2 cells. Methods Tumor microenvironment was simulated by decreasing the VEGF concentration with bevacizumab monoantibody. Expression of VEGF2 in HepG2 cells was detected by RT-PCR, ELISA and Western blot, respectively. Results The VEGF concentration was significantly decreased （P 〈 0.05） and the soluble VEGFR2 concentration was significantly increased in medium （P 〈 0.05）. No significant change occurred in the VEGF and VEGFR1 expression level （P 〉 0.05） whereas the expression of VEGFR2 was significantly upregulated in HepG2 cells （P 〈 0.05）. Conclusion Bevacizumab up-regulates the expression of VEGF2 in HepG2 cells.

关 键 词：贝伐珠单抗 肝癌细胞HEPG2 血管内皮生长因子 血管内皮生长因子受体2 

分 类 号：R342[医药卫生—基础医学]