IL-1β对ATDC5成软骨分化细胞miR-455-3p的影响  

IL-1 beta induces expression of miR-455-3p in differentiated ATDC5 cells partly via NF-kappa B signaling

作　　者：黄广鑫[1] 张志奇[1] 侯昌禾[1] 黄志宇[1] 王秉钧[1] 余宝禧 邬培慧[1] 傅明[1] 杨子波[1] 廖威明[1] 

机构地区：[1]中山大学附属第一医院关节外科,广州510080

出　　处：《中华关节外科杂志（电子版）》2014年第2期45-50,共6页Chinese Journal of Joint Surgery(Electronic Edition)

基　　金：国家自然科学基金项目(81171759;81201388);广东省科技计划项目(2011B031300012)

摘　　要：目的检测IL-1β对ATDC5成软骨分化细胞miR-455-3p表达的影响,探索miR-455-3p在骨关节炎中的作用。方法诱导ATDC5细胞成软骨分化后,予10 ng/ml的IL-1β刺激,在刺激4、12、24、48 h时应用实时荧光定量PCR检测miR-455-3p、C/EBPβ和软骨特征性标记物的表达情况;并利用抑制剂IKK-NBD阻断NF-κB通路后,应用实时荧光定量PCR检测IL-1β作用下miR-455-3p的表达水平。结果在IL-1β作用下的ATDC5成软骨分化细胞中miR-455-3p、C/EBPβ和软骨退变标记物(MMP13、ADAMTS5)均上调,而软骨基质合成标记物(ACAN、COL2A1、SOX9)则下调,且后期更为明显;而IKK-NBD可抑制IL-1β诱导的miR-455-3p表达。结论 IL-1β可上调ATDC5成软骨分化细胞miR-455-3p的表达水平,且受NF-κB通路的调节。Objective To investigate the effects of IL-1 beta （ IL-1β） on the expression of miR-455-3p in the differentiated ATDC5 cells.Methods After chondrogenic differentiation , ATDC5 cells were treated with 10ng/ml recombinant murine IL-1βfor different time length.Additionally, a NF-kappaB inhibitor IKK-NBD was added to the cell culture before the IL-1βtreatment.The expression of miR-455-3p, C/EBP βand the genes related to cartilage were detected by quantitative real-time PCR.Results＆amp;nbsp;The IL-1β-treat differentiated ATDC5 cells increased the expression of miR-455-3p, C/EBP β, MMP13 and ADAMTS5, while decreased the expression of ACAN , COL2A1 and SOX9 especially in the late stage. IL-1β-induced up-regulation of miR-455-3p was inhibited by IKK-NBD.Conclusion The expression of miR-455-3p in the differentiated ATDC5 cells can be induced by IL-1βand NF-kappa B is involved in the up-regulation.

关 键 词：微RNAS 白细胞介素11β NF—κB 骨关节炎 

分 类 号：R684.3[医药卫生—骨科学]