唑来膦酸钠在抑制钛颗粒诱导骨溶解中作用的体外实验研究  

Inhibitory effects of zoledronate on titanium particle-induced osteolysis in vitro

作　　者：张茂林[1] 丁悦[1] 张驰[1] 李长川[1] 傅光涛 

机构地区：[1]中山大学孙逸仙纪念医院骨外科,广州510120

出　　处：《中华关节外科杂志（电子版）》2014年第2期54-57,共4页Chinese Journal of Joint Surgery(Electronic Edition)

基　　金：国家自然科学基金项目(81272020);中山大学青年培育项目(10ykpy22)

摘　　要：目的：研究唑来膦酸钠（ ZOL）对钛颗粒诱导的骨溶解的影响。方法分离6～8周C57BL/6J小鼠长骨中的前体破骨细胞（ OCP）并分为6组，A组：OCP＋细胞培养液，B组：OCP＋巨噬细胞集落刺激因子（M-CSF）＋NF-κB 受体活化因子配体（RANKL）＋细胞培养液，C组：OCP＋钛颗粒＋细胞培养液，D组：OCP＋上清液（钛颗粒刺激巨噬细胞24 h后上清液）＋细胞培养液，E组：OCP＋M-CSF＋RANKL＋ZOL＋细胞培养液，F组：OCP＋上清液＋ZOL＋细胞培养液。每组细胞分别接种在玻璃盖玻片、皮质骨磨片和含骨检测表面的96孔板上，10 d后检测玻璃盖玻片上细胞抗酒石酸磷酸酶（ TRAP）的表达及皮质骨磨片上骨吸收陷窝的形成，并以骨检测表面的骨吸收面积为指标比较各组破骨细胞的骨吸收活性。结果 B组、D组、E组和F组的OCP均能分化为能被TRAP染色成阳性的破骨细胞并形成骨吸收陷窝，其余组均未发现TRAP染色阳性的破骨细胞和骨陷窝。加入ZOL的F组骨吸收面积（5.54％±1.25％）较D组（10.34％±1.69％）明显减少，差异具有统计学意义（t＝5.61，P＜0.01）。结论在体外实验中钛颗粒并不能直接刺激前体破骨细胞向破骨细胞转化；唑来膦酸钠可以抑制钛颗粒诱导的骨溶解作用。Objective To investigate the effects of zoledronate on the titanium particle-induced osteolysis.Methods The osteoclast precursors （OCP） were isolated from the long bones of six-week-old＆amp;nbsp;C57BL/6J mice and assigned into six groups with different components as follows ： group A, only the culture medium; group B, the macrophage colony stimulating factor （M-CSF）, the receptor activator of nuclear factor kappa ligand （ RANKL） and the culture medium; group C, the titanium particles and the culture medium; group D, the supernatant （ from the cultured mouse macrophages stimulated by the titanium particles for 24 h） and the culture medium;group E, the M-CSF, RANKL, ZOL and the culture medium;group F, the supernatant, ZOL and the culture medium.After 10 days, the coverslips were stained by the tartrate-resistant cultured acid phosphatase （ TRAP） , and the activity of the osteoclast cells was detected by the area of osteo-assay surface resorption .Results The TRAP positive multinucleated cells and the resorpion lacunae were observed in group B , group D, group E and group F.In group F （5.54%±1.25%）, the area of the resorption lacunae was smaller than that in group D （10.34% ± 1.69%, t=5.61, P〈0.01）.Conclusion The zoledronate can inhibit the activation of the osteoclasts induced by the titanium particles in vitro .

关 键 词：唑来膦酸钠 破骨细胞 骨溶解 钛颗粒 

分 类 号：R687.4[医药卫生—骨科学]