枣实蝇4个地理种群的线粒体Cytb、基因序列分析  

Sequence Analysis of Mitochondrial Cytb Gene of Four Geographic Populations of Carpomya vesuviana

作　　者：程晓甜[1] 阿地力·沙塔尔[1] 张伟[2] 李新泉[1] 

机构地区：[1]新疆农业大学林学与园艺学院新疆教育厅干旱区林业生态与产业技术重点实验室,乌鲁木齐830052 [2]新疆出入境检验检疫局技术中心,乌鲁木齐830083

出　　处：《林业科学》2014年第3期144-150,共7页Scientia Silvae Sinicae

基　　金：新疆维吾尔自治区科技计划项目“特色林果重大病虫持续高效绿色防控技术研究”(201130102-3);新疆维吾尔自治区重点学科森林培育资助项目;新疆维吾尔自治区林业有害生物防治检疫局项目“枣实蝇快速检测和监测技术研发”

摘　　要：新人侵重大检疫性害虫枣实蝇（Carpomya vesuviana）于2007年首次在吐鲁番地区发现，对该地区枣（Zizyphus jujuba）产业造成了毁灭性损失，且疫情有向其他地区扩散的趋势。枣实蝇原产印度，现已扩散至阿富汗、塔吉克斯坦、泰国、意大利等国家（张润志等，2007）。枣实蝇以成虫产卵和幼虫蛀食危害枣果，成虫产卵于果实表面，致使产卵孔周围组织发育停止，形成凹陷或畸形。In this study, PC R amplification and sequencing techniques were used to compare and analyze mitochondrial Cytb gene sequences of four geographic populations （KSS, Turpan, HSX and HMS） of Carpomya vesuviana , to explore the genetic relationship among them. 469 bp fragments were obtained. The composition,specific site and base transition/ transversion frequency of the partial sequences of Cytb gene were analyzed. The frequencies of codon usage were calculated and the phylogenetic tree was reconstructed. The results showed that KSS population has a relatively far genetic relationship with Turpan population, HSX population and HMS population.

关 键 词：枣实蝇 CYTB 进化树 亲缘关系 

分 类 号：Q965[生物学—昆虫学]