HPLC法测定中性腹膜透析液(乳酸盐)中葡萄糖的含量  

Determination of glucose in neutral peritoneal sialysis solution (lactate) by HPLC

作　　者：滕霞 唐素华 

机构地区：[1]青岛海大科技制药有限公司,山东青岛266101 [2]青岛华仁药业股份有限公司,山东青岛266101

出　　处：《药学研究》2014年第5期274-275,281,共3页Journal of Pharmaceutical Research

摘　　要：目的：建立高效液相色谱法测定中性腹膜透析液（乳酸盐）中葡萄糖含量的方法。方法采用 Waters Sugar ParkⅠ色谱柱（6.5 mm ＆#215;300 mm，6.5μm），流动相为水，流速：0.5 mL·min -1，柱温：80℃，示差检测器设置温度：40℃，进样量：20μL。结果葡萄糖在1.13378~6.80268 mg·mL -1范围内呈良好的线性关系（r =1.0000）。含1.5%葡萄糖的中性腹膜透析液（乳酸盐）平均回收率为99.1%，RSD =0.4%（n =9）；含2.5%葡萄糖的中性腹膜透析液（乳酸盐）平均回收率为100.1%，RSD =0.2%（n =9）；含4.25%葡萄糖的中性腹膜透析液（乳酸盐）平均回收率为100.2%，RSD =0.3%（n =9）。结论本方法简便、快速、准确，可用于中性腹膜透析液（乳酸盐）的质量控制。Objective To establish an HPLC method for the determination of glucose in neutral peritoneal sialysis so-lution（lactate）. Methods Waters Sugar Park I column（6. 5 mm × 300 mm,6. 5 μm）,the mobile phase was water with the flow rate of 0. 5 mL·min - 1. The column temperature was 80 ℃. The set temperature of differential detector was 40 ℃ and the sample size was 20 μL. Results The linear range of glucose was 1. 133 78 ~ 6. 802 68 mg·mL - 1（r = 1. 000 0）. The average recovery of containing 1. 5% glucose in neutral peritoneal sialysis solution（lactate）was 99. 1% ,RSD = 0. 4 %（n =9）;The average recovery of containing 2. 5% glucose in neutral peritoneal sialysis solution（lactate）was 100. 1% ,RSD =0. 2%（ n = 9）;The average recovery of containing 4. 25% glucose in neutral peritoneal sialysis solution（ lactate）was 100. 2% ,RSD = 0. 3%（n = 9）. Conclusion The method was simple,rapid,accurate and can be used for neutral perito-neal sialysis solution（lactate）for quality control .

关 键 词：高效液相色谱法 中性腹膜透析液(乳酸盐) 葡萄糖 含量测定 

分 类 号：R927.2[医药卫生—药学]