鸡鲍氏3型志贺菌脂多糖及O抗原多糖的提纯与鉴定  

Extraction and Identification of Lipopolysaccharide and O-polysaccharide from Chicken S.boydii Type 3

作　　者：魏亚鹏[1] 郭会娟[1] 张美玲[1] 常洪涛[1] 刘红英[1] 王川庆[1] 王新卫[1] 杨霞[1] 陈陆[1] 赵军[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国畜牧兽医》2014年第5期133-136,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30972187)

摘　　要：为获得高纯度的鸡鲍氏3型志贺菌脂多糖(lipopolysaccharide,LPS)及其亚单位O抗原多糖(O-polysaccharide,OPS),采用改良热酚水法提取鸡鲍氏3型志贺菌的LPS,并联合使用葡聚糖凝胶层析得到高纯度的LPS;采用酸水解法联合葡聚糖凝胶层析获得高纯度的O-PS。生化方法检测结果显示所提纯LPS和O-PS纯度高,兔回肠襻试验结果显示所提纯的LPS活性好。本试验结果表明提取的LPS和O-PS可进行特异性研究及制备有效的候选疫苗。To obtain LPS and its subunit O- PS with high purity from chicken S. boydii type 3, LPS from chicken Shigella was extracted with modified hot phenol-water method, high purified LPS was obtained through sepbadex gel chromatography, O-PS was obtained by combination with acid hydrolysis method and sephadex gel chromatography. Biochemical assay showed that the purified LPS and O-PS with high purity, rabbit ideal loop experiment demanstrated that there was high activity in puri fled LPS. The result indicated that extracted LPS and O-PS could be used in specific pathogenesis research and developed as ef fective vaccine candidates.

关 键 词：鸡鲍氏3型志贺菌 LPS O-PS 提纯 

分 类 号：Q78[生物学—分子生物学]