绵羊瘤胃内纤维降解菌的分离鉴定  

Isolation and Identification of the Cellulose-degrading Bacteria Strains from Sheep Rumen

作　　者：那仁图雅[1] 周非帆 杨金丽[1] 侯先志[1] 王海荣[1] 

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018

出　　处：《中国畜牧兽医》2014年第5期142-146,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31101737)

摘　　要：试验从蒙古绵羊瘤胃中分离出3株厌氧细菌,通过细菌纤维降解性试验发现3株菌皆具有纤维降解能力;通过革兰氏染色试验发现3株菌均为革兰氏阳性菌;通过生理生化试验和16SrDNA基因序列测定及同源性分析,最终确定分离的3株菌分别为牛链球菌(Streptococcus bovis)、婴儿链球菌(Streptococcus infantarius)和粪肠球菌(Enterococcus faecium),编号为E1、E2和E3。Three strains of anaerobic bacteria were isolated from the rumen of the Mongolian sheep. The bacterial cellulose degradation detection test showed that all of the bacteria have fiber degradation ability. All of the bacteria were gram-positive bacteria according to the gram stain test. After the physiological and biochemical test, and determination of 16S rDNA gene se quences and the homology analysis,the three strains of bacteria turned out to be Streptococcus boris ,Streptocococcus infantarius and Enterococcus faecium, and were named as El, E2 and E3, respectively.

关 键 词：纤维降解菌 厌氧 分离 鉴定 

分 类 号：S826[农业科学—畜牧学]