紫外分光光度法测定甲钴胺注射液含量  

Determination of Mecobalamin injection by Ultraviolet Spectraphotometr

作　　者：雷晓雪[1] 陈文芝[1] 张高平[1] 王丽楠[1] 

机构地区：[1]扬子江药业集团南京海陵药业有限公司,江苏南京210049

出　　处：《亚太传统医药》2014年第9期18-19,共2页Asia-Pacific Traditional Medicine

摘　　要：目的：建立紫外分光光度法（UV）快速测定甲钴胺注射液含量.方法：首次以水为溶剂,在266nm处测定吸光度并计算注射液中甲钴胺含量.结果：甲钴胺在3～60 μg/mL浓度范围内与吸光度呈良好的线性关系,回归方程为：Y=0.013 66X＋0.001 68,r=0.999 97,平均回收率为100.3％（n=9,RSD=0.3％）.结论：该方法简便、快速、准确、专属性强、重现性好,可用于甲钴胺注射液的质量控制.Objective：To establish a method for the determination of ropivacaine mesylate injection by ultraviolet spectrophotometry. Methods： H20 was taken as solvent and detected at 266nm, The content of ropivacaine mesylate was measured and calculated. Results：The linear equation was Y=0. 013 66X＋0. 001 68,r=0. 999 97,and the calibration curve was linear in the range of 3- 60μg/mL. The average recovery was 100.3% and RSD was 0.3% （n= 9）. Conclusion： The UV method was simple, accurate, specific, reproducible, and it can be used for determination of mecobalamin injection.

关 键 词：紫外分光光度法(UV) 甲钴胺注射液 含量测定 

分 类 号：R927.2[医药卫生—药学]