基于微量量热法的寒热中药对大肠杆菌生长热谱曲线的影响  

A Microcalorimetric Research on the Effect of Traditional Chinese Medicine on the Growth and Metabolism of E. coli

作　　者：高誉珊[1] 郑丰杰[1] 李鑫[1] 许红[1] 张淑静[1] 李宇航[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《云南中医学院学报》2014年第2期10-13,33,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：教育部"211"工程三期建设项目;北京中医药大学创新团队项目(2011-CXTD-04)

摘　　要：目的观察典型寒热中药黄连、制附子对大肠杆菌生长代谢作用的影响,探讨微量量热法用于中药寒热差异及量化研究的可行性。方法采用微量量热技术,测定大肠杆菌在不同浓度寒热中药水煎液干预下的生长热谱曲线,分析其热力学参数。结果黄连、制附子均可影响大肠杆菌产热效应,其效应影响与药物浓度具有正相关关系;黄连组的热焓值明显低于对照组,制附子组的热焓值明显高于对照组。结论微量量热技术是从生物体生命周期及能量代谢角度,观察中药寒热效应差异的客观化手段之一。Objective To investigate the effect of berberis,aconite tuber,on the growth and metabolism of E. coli and to explore the feasibility of applying microcalorimetry to quantitative researches on the differences between coldness and heatiness of herbal medicine. Methods Microcalorimetric techniques were applied to evaluate thermal spectral profiling on the growth of E. coli at different concentrations of water decoctionand analyze the thermodynamic parameters. Results This Chinese Medicines both significantly influenced the thermogenic effects of E. coli. A positive correlation is found between the concentration of the drug and thermogenic effects. compared with the control group,the enthalpy value of the berberis group was significantly reduced. Meanwhile the enthalpy value of the aconite tuber group was significantly higher than control group. The inhibiting effect to the E. coli showed a significant increase in aconite tuber group and a trend toward lower inhibiting effect in berberis group. Conclusion The microcalorimetric techniques can be applied in quantitative classification of Chinese herbal coldness and heatiness.

关 键 词：微量量热法 中药四气 黄连 附子 大肠杆菌 

分 类 号：R378.2[医药卫生—病原生物学]