正交试验法优选马钱子总生物碱提取工艺  

Optimization of Extraction Technology of Total Alkaloids of Semen Strychni by Orthogonal Test

作　　者：张文平[1] 张贵华[1] 柯瑾[1] 马云淑[1] 

机构地区：[1]云南中医学院,云南昆明650500

出　　处：《云南中医学院学报》2014年第2期14-16,共3页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(30960517);云南省教育厅科学研究基金(2012C040;2011Y398)

摘　　要：目的优选马钱子中士的宁与马钱子碱的提取方法与工艺。方法在单因素试验的基础上,以高效液相测定的士的宁与马钱子碱总量为指标,以乙醇浓度、乙醇用量及回流时间为影响因素,采用正交试验法优选士的宁与马钱子碱提取工艺。结果优选的最佳提取工艺为50%乙醇10倍量,回流提取3次,每次2h。结论验证实验表明该工艺合理、稳定可行。Objective To optimize extraction process conditions of Semen Strychni. Methods With the content of Strychnine and brucine as the target,based on the single factor experiment,extraction technology of Semen Strychni was optimized by L9（34） orthogonal test,taking the concentration of ethanol,ethanol dosage and extraction time as investigated factors. Results Optimum extraction technology was：extracted 3 times with 10 times the amount of 50%ethanol,2 hours each time. Conclusion This optimized extraction technology was simple and reasonable and it was stable.

关 键 词：马钱子 总生物碱 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]