新疆地区冠心病秽浊痰阻证动物模型的实验研究  

Study on Animal Model of Xinjiang Coronary Heart Disease with Phlegm-turbidity Syndrome

作　　者：王昕宇[1] 杨剑[2] 安冬青[1] 

机构地区：[1]新疆医科大学中医学院,新疆乌鲁木齐830011 [2]新疆医科大学附属中医医院,新疆乌鲁木齐830011

出　　处：《中国中医急症》2014年第4期585-588,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：新疆维吾尔自治区自然科学基金(2010211A24);新疆名医名方与特色方剂学实验室开放课题(XJDX0910-2009-04)

摘　　要：目的通过人工气候箱结合喂养高脂饲料并腹腔注射维生素D3,建立新疆地区冠心病秽浊痰阻证大鼠模型。通过组织切片、血清指标,摸索模型建立条件。方法按RCT原则,将20只SD大鼠分为空白对照组、秽浊痰阻证模型组,每组10只。空白对照组正常饮水喂基础饲料;秽浊痰阻证模型组正常饮水喂高脂饲料并腹腔注射维生素D3,同时每日固定11 h在人工气候箱寒燥条件下饲养。实验第13周,行大鼠主动脉瓣至髂肾动脉分支处动脉及肝脏组织切片检查;测体质量、血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋胆固醇、极低密度脂蛋白胆固醇、血清白细胞介素6、肿瘤坏死因子-α。结果两组大鼠体质量、血清总胆固醇、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇、IL-6、TNF-α差异均有统计学意义(P<0.05),而三酰甘油、高密度脂蛋白胆固醇差异无统计学意义(P>0.05);动脉血管壁内膜下见无定形物质沉积,有泡沫样细胞;肝组织外周出现中度肝脂肪变性。结论冠心病秽浊痰阻证大鼠模型,基本表现出新疆地区冠心病秽浊痰阻证辨证要点,初步推测造模方法具有一定可行性。Objective： To establish animal model of Xinjiang coronary heart disease with phlegm-turbidity syn- drome by combined treatment of artificial climate chamber,high-fat diet,abdominal injection of vitamin D3,and to explore animal model conditions by biopsy and serum index. Methods： By RCT principle, 20 SD rats were divided into normal control group and phlegm-turbidity syndrome group, 10 rats in each group. Normal control group was fed the basis diet;phlegm-turbidity syndrome group was fed the high-fat diet,injected with vitamin D3,and fixed 11 hours a day in the artificial climate chamber of cold dry conditions.13 weeks later,tissue sec- tions of aortic renal artery branch to iliac artery and liver were checked. Then weight, total cholesterol in serum, triglycerides,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,extremely low density lipoprotein cholesterol,interleukin-6in serum, and tumor necrosis factor-or were measured. Result： Compared with normal control group,body weight,total cholesterol in serum,low-density lipoprotein cholesterol,extremely low density lipoprotein cholesterol,serum interleukin-6,and tumor necrosis factor-eL in model group had statistical differences （P〈 0.05）. In aspects of triglycerides and high-density lipoprotein cholesterol,there were no signifi- cant differences （P〉 0.05）. There were amorphous materials deposited under artery wall intima. Foam-like cells and moderate hepatic steatosis in liver tissue periphery were observed. Conclusions： Animal model demonstrates the basic clinical characters of Xinjiang coronary heart disease with phlegm-turbidity syndrome so modeling method is initially speculated of certain feasibility.

关 键 词：冠心病秽浊痰阻证大鼠模型 血脂 组织切片 白细胞介素6 肿瘤坏死因子α 

分 类 号：R285.5[医药卫生—中药学]