生物活性化合物的靶点鉴别方法  

Methods for target identification of bioactive compounds

作　　者：陈林林[1,2] 厉建中[2] 张俊平[1,2] 

机构地区：[1]福建中医药大学,福州350122 [2]第二军医大学药学院生化药学教研室,上海200433

出　　处：《国际药学研究杂志》2014年第2期172-177,共6页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(81270508)

摘　　要：生物活性化合物作用靶点的确认是化学生物学和药物开发中的关键问题之一。随着科技的进步与发展,许多靶点鉴别的方法和技术已经被报道。目前的靶点鉴别方法主要有两大类:亲和色谱为基础的直接法,通过检测药物与其靶点的结合来鉴别靶点;非亲和为基础的间接法,通过生理学反应或生物化学标记而推断药物的靶点或作用途径。本文主要围绕这两类靶点鉴别方法进行综述。Target identification of bioactive compounds is one of the key issues in chemical biology and drug discovery. With the development of science technology, a variety of methods and technologies for target identification have been reported. They can be fundamentally categorized into two approaches： the direct method based on affinity chromatography which is mainly to detect the combi- nation of drug and targets, and the indirect one which is mainly to predict the drug target and action mechanism by physiological reac- tion and biochemical marker. This review aims to describe the two approaches for target identification.

关 键 词：靶点鉴别 亲和色谱 对比分析 

分 类 号：TQ464[化学工程—制药化工]