奥伐尼对芥子气所致皮肤损伤的保护作用  

Protective effect of olvanil against sulfur mustard-induced cutaneous injury

作　　者：郑盼[1] 肖智勇[1] 梅逸舟 刘峰[1] 程军平[1] 尹纪业[1] 周文霞[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《国际药学研究杂志》2014年第2期206-210,共5页Journal of International Pharmaceutical Research

基　　金：国家"重大新药创制"科技重大专项资助项目(2013ZX09J13103-01B)

摘　　要：目的研究奥伐尼对芥子气所致皮肤损伤的保护作用。方法采用32和128 g/L芥子气5μl于KM小鼠右耳内侧染毒,建立两种损伤程度的小鼠耳郭染毒皮肤损伤模型。在32 g/L芥子气染毒模型中,染毒前20 min于小鼠右耳内侧涂抹奥伐尼0.125、0.25和0.5 mg/耳,或染毒前30 min灌胃给予吲哚美辛10 mg/kg,染毒24 h后,观察各组耳郭肿胀程度,制备耳郭皮肤切片观察皮肤组织病理变化。在128 g/L芥子气染毒模型中,小鼠右耳给予奥伐尼0.25 mg/耳或灌胃给予吲哚美辛10 mg/kg,染毒24 h后观察各组耳郭肿胀程度。结果奥伐尼可显著降低32 g/L芥子气所致耳郭肿胀,并显著改善皮肤炎症、坏死等病理变化,但对128 g/L芥子气所致耳郭肿胀无明显影响。结论奥伐尼对低剂量芥子气染毒所致皮肤损伤具有显著的保护作用。Objective To study the protective effects of olvanil on sulfur mustard （SM）-induced cutaneous injury. Methods To establish mouse ear model of SM-induced cutaneous injury, KM mice right ears were exposed to 5 μl of 32 g/L or 128 g/L SM, re- spectively. Then we evaluated the protective effect of olvanil against SM-induced cutaneous injury with these two concentrations. For mice exposed to 32 g/L SM, olvanil （0. 125, 0. 25 and 0. 5 rag/ear, topically） or indometacin （indomethaein, 10 mg,/kg, po） were administrated 20 min or 30 min before SM exposure, respectively. After 24 h SM exposure, ear tissue was harvested and weighted, and the histopathologieal analysis was conducted. Similarly, for mice exposed to 128 g/L SM, olvanil （0. 25 mg/ear, topically） or indom- ethaein （ 10 mg/kg, po） were administrated, and ear tissue was harvested and weighted. Results Olvanil significantly inhibited 32 g/L SM-indueed ear edema, cutaneous inflammation and necrosis, while no influence were observed on 128 g/L SM-indueed cutaneous injury. Conclusion Olvanil shows remarkable protective effect on cutaneous injury induced by SM of low concentration.

关 键 词：芥子气 皮肤损伤 吲哚美辛 奥伐尼 

分 类 号：R965[医药卫生—药理学]