不同产地淫羊藿总多糖的理化性质比较  

Comparision of the physicochemical properties of Epimedium polysaccharides from different producing areas in China

作　　者：麻浩[1] 张亚欣[1] 张贵强[1,2] 文松[1,2] 李帅[1] 王玉霞[1] 单俊杰[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]贵州大学生命学院,贵阳550025

出　　处：《国际药学研究杂志》2014年第2期249-253,共5页Journal of International Pharmaceutical Research

基　　金：国家"十二五"新药创制重大专项课题(2012ZX09301001-003);国家"十二五"科技支撑课题(2011BAD26B02)

摘　　要：目的研究和比较全国不同省份或地区淫羊藿药材总多糖的理化性质,为淫羊藿佐剂多糖的开发提供药材产地选择依据。方法采用50℃条件进行水浸提、醇沉、透析和冷冻干燥获得总多糖。采用苯酚-硫酸法测定糖含量,间羟联苯法测定糖醛酸含量,凝胶渗透色谱法测定多糖相对分子质量分布,糖醇乙酸酯衍生-气相色谱法测定单糖组成。结果不同产地的淫羊藿总多糖理化性质存在明显差异,其中陕西、湖南和吉林淫羊藿总多糖的得率较高,四川、湖南和吉林淫羊藿总多糖的糖含量较高,湖南、吉林和河南淫羊藿总多糖的糖醛酸含量较高,总多糖的相对分子质量分布相近,单糖组成差异较大,特别是半乳糖、甘露糖和阿拉伯糖的含量差异较大。结论不同产地的淫羊藿药材的总多糖成分存在较大差异,可能与种属基因不同、生长环境不同有关。Objective To investigate the physicochemical properties of Epimedium polysaccharides（EP） from different produ- cing areas in China to provide experimental support for selecting qualified herbal materials for specified product development. Methods The leaves of Epimedium plants were extracted with water at 50℃, and the aqueous-extract was precipitated with ethanol. The precip- itates were dissolved, dialyzed and lyophilized to obtain total polysaccharides, EP. Total sugar and uronic acid contents of the EP were determined by the phenol-sulfuric acid and the m-hydroxyl biphenyl methods, respectively. Molecular weights of the EP were determined by gel filtration chromatography, andmonosaccharide compositions of the EP were analyzed with the alditol acetates by gas chromatography. Results Total polysaccharides in the Epimedium leaves were significantly different in the leaves from different area of China. Yields of the EP were higher in the leaves from Shannxi, Hunan and Jilin provinces, but the total sugar contents were higher in the EP from the leaves from Sichuan, Hunan and Jilin provinces, and the uronic acid contents in the EP higher in those from Hunan, Jilin and Henan provinces. Molecular weight distributions were similar in all of the EP, but the monosaccharide compositions of the EP were different mainly in the molar ratios of mannose, glucose and galactose. Conclusion There were significant differences in the physicochemical properties of the EP from the Epimedium plants in different areas of China, which may originate from the different genes of varied species and/or the different habitats of the plants.

关 键 词：淫羊藿 产地 总多糖 理化性质 

分 类 号：R284[医药卫生—中药学]