Identification of the Pathogen of Wufeng Maryland Tobacco Hollow Stalk  

作　　者：Zhang Shuguang Zhang Min Sun Hongxu Liu Shenggao Wen Guanghong 

机构地区：[1]Hubei Three Gorges Polytechnic [2]Yichang Tobacco Company

出　　处：《Plant Diseases and Pests》2014年第1期21-22,共2页植物病虫害研究（英文版）

基　　金：Supported by Tobacco Research Project of Hubei Tobacco Company(2011-13);Scientific and Technological Project of Yichang City(A2011-201)

摘　　要：The morphological characteristics, pathogenicity and biological characteristics of the pathogen isolated from Maryland tobacco in Wufeng County of Hubei Province were studied in the laboratory. After cultivation on LB medium at 28℃ for48 h, the colonies were light yellow, circular or amorphous, translucent, with smooth and slightly concaved surface and neat edge. Observation under electron microscope showed that the isolates was red-shaped, decapsulated and gram-nega- tive in the size of （0.5 - 1.0） μm × （2.2 -3.0） μm, with 2 -8 flagella. After cultivation on CVP medium at 28 ℃ for 48 h, the colonies were sunken like a cup. The pathogen was able to grow in 5% NaCl medium at 37 ℃, and produced acid through glucose fermentation; it was facuhative anaerobic, and could use citrates, but could not produce indole. It was preliminarily confirmed that the pathogen causing Wufeng Maryland tobacco hollow stalk was Pectobacterium carotovorum subsp. carotovorum.The morphological characteristics, pathogenicity and biological characteristics of the pathogen isolated from Maryland tobacco in Wufeng County of Hubei Province were studied in the laboratory. After cultivation on LB medium at 28℃ for48 h, the colonies were light yellow, circular or amorphous, translucent, with smooth and slightly concaved surface and neat edge. Observation under electron microscope showed that the isolates was red-shaped, decapsulated and gram-nega- tive in the size of （0.5 - 1.0） μm × （2.2 -3.0） μm, with 2 -8 flagella. After cultivation on CVP medium at 28 ℃ for 48 h, the colonies were sunken like a cup. The pathogen was able to grow in 5% NaCl medium at 37 ℃, and produced acid through glucose fermentation; it was facuhative anaerobic, and could use citrates, but could not produce indole. It was preliminarily confirmed that the pathogen causing Wufeng Maryland tobacco hollow stalk was Pectobacterium carotovorum subsp. carotovorum.

关 键 词：Wufeng Maryland tobacco TOBACCO Hollow stalk Identification of pathogen Pectobacterium carotovorum subsp. Carotovorum 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]