染料木素抑制大鼠离体胃肠平滑肌收缩活动的研究  

Inhibitory effects of genistein on contractile activity of isolated gastrointestinal smooth muscle in rats

作　　者：李长兴[1] 李红芳[2] 张立雪[3] 豆兴成[2] 田治峰[2] 

机构地区：[1]青海大学医学院人体解剖学教研室,青海西宁810001 [2]兰州大学基础医学院生理学教研室,甘肃兰州730000 [3]西北民族大学医学院,甘肃兰州730000

出　　处：《重庆医学》2014年第13期1543-1545,共3页Chongqing medicine

基　　金：青海大学医学院中青年科技基金资助项目(2013-RY-6);甘肃省科技厅自然科学基金资助项目(1010RJZA111)

摘　　要：目的观察染料木素对大鼠离体胃肠平滑肌收缩活动的影响,并探讨其作用机制。方法取大鼠胃肠平滑肌条安置在恒温灌流肌槽中,记录其收缩活动,同时做CaCl2的量效曲线。结果染料木素能降低胃肠平滑肌条的张力,减小其收缩波平均振幅。酚妥拉明、左旋-NG-硝基精氨酸、格列本脲、SQ22536可部分阻断染料木素对大鼠离体胃肠收缩活动的抑制作用。1.00mol/L的染料木素对CaCl2的量效曲线无影响,50.0mol/L染料木素使CaCl2的量效曲线右移。结论染料木素对大鼠胃肠平滑肌条收缩活动具有抑制作用。Objective To investigate the effects of genistein（Gen）on contractile activity of gastrointestinal smooth muscle of rat and explore its underlying mechanisms.Methods Gastrointestinal smooth muscle strips were taken out from rat.Each isolated muscle strip was put in a tissue chamber which contains 5 mL krebs solution and the motility of muscle strips was recorded simulta-neously.At the same time the CaCl2 concentration-response curves were done.Results Genistein reduced the resting tension of gas-trointestinal smooth muscle strips and decreased mean contractile amplitude of gastrointestinal smooth muscle strips,which were dose-dependent.Phentolamine,l-NG-nitroarginine,glibenclamide,SQ22536 partially blocked the inhibition of puerarin on the con-tractile activity of the rat gastric body.Genistein（1.00μmol/L）had no significant effect on calcium-dependent contraction,while genistein（50.00μmol/L）markedly prevented contractile effects of CaCl2 on body of stomach.Conclusion Genistein has inhibitory action on the isolated gastrointestinal smooth muscle strips of rats.

关 键 词：染料木素 肌 平滑 胃肠活动 酚妥拉明 左旋-NG-硝基精氨酸 格列本脲 SQ22536 

分 类 号：R285.5[医药卫生—中药学]