顶空气相色谱法快速测定水果中仲丁胺的残留量  

Rapid Determination of Sec-butylamine Residue in Fruits by Headspace Gas Chromatography

作　　者：韩梅[1] 胡莉[1] 杨晓凤[1] 吴显平[2] 

机构地区：[1]四川省农业科学院分析测试中心,四川成都610066 [2]成都医学院,四川成都610500

出　　处：《江西农业学报》2014年第5期98-100,共3页Acta Agriculturae Jiangxi

摘　　要：利用仲丁胺在水溶液中受热挥发的特性，建立了顶空气相色谱法快速测定多种水果中仲丁胺残留量的方法。用水提取样品中仲丁胺，提取液离心后，顶空进样，Rtx—17毛细管柱（30m×0．25mm，0．25μm）分离，氢火焰离子化检测器（FID）测定。仲丁胺在0．01—2．00mg／L范围内，峰面积与质量浓度呈线性关系，相关系数为0．9996，6种水果的仲丁胺平均回收率为70．22％-115．30％，相对标准偏差为1．1％-11．9％，检出限为0．01mg／kg。该方法操作过程简单、快速、灵敏，满足仲丁胺残留分析的要求，可用于某些水果中保鲜剂仲丁胺残留量的测定。A method has been constructed for the rapid determination of sec - butylamine residue in various fruits by means of the headspaee gas chromatography based on the volatility of see - butylamine in heated aqueous solution. Sec - butylamine in sam- ples was extracted by water, and the obtained extracting solution was centrifuged. Then it was analyzed by using headspaee sampler in gas chromatography with hydrogen flame ionization detector （FID）, and was separated by a Rtx - 17 capillary column （30 m ×0.25mm,0.25μm）. There was a linear relationship between the peak area and the mass concentration of see - butylamine from 0. 01 to 2.0 mg/L with the correlation coefficient of 0. 9996. The average recoveries of sec - butylamine in 6 kinds of fruits varied from 70.22% to 115.30%, the relative standard deviation was 1.1% ~ 11.9% , and the detection limit of the method was O. 01 mg/kg. This method was simple, rapid and sensitive in application, could meet the requirements of see - butylamine residue analy- sis, and could be used to determine the residual amount of the preservative see - butylamine in some fruits.

关 键 词：顶空气相色谱法 仲丁胺 水果 检出限 

分 类 号：O657.72[理学—分析化学]