LAMP技术在食源性病原微生物检测中的应用  

The Application of LAMP Technology in Detection for Forborne Pathogenic Microorganisms

作　　者：陈传[1] 凌霄[2] 郭震[1] 邵筱[1] 张如胜 魏泉德 

机构地区：[1]广东医学院,中国广东湛江524023 [2]广东医学院第二附属医院,中国广东湛江524023 [3]珠海市疾病预防与控制中心,中国广东珠海519000

出　　处：《生命科学研究》2014年第2期140-144,共5页Life Science Research

基　　金：2011年湛江市科技攻关计划项目(2011C3101005)

摘　　要：针对常见引起食物中毒细菌的特异保守基因设计环介导恒等温扩增(LAMP)引物,并以此套引物建立食源性病原微生物的检测方法。特异性及灵敏性试验显示,LAMP法最低检出限为10-5,Real-time LAMP最低检出限为10-7,而普通PCR的检出下限仅为10-3;且LAMP全部反应在1 h内完成;LAMP反应结果可通过肉眼直接观测判定结果。LAMP快速检测常见食物中毒菌的方法初步建立,同时结合Real-time LAMP,可提供更准确的检测结果并可实现仪器在线式实时监测。This experiment target gene primers were designed for common food poisoning bacteria, and thus sets the primers create a pathogenic microorganism diagnosis technology based on LAMP method. The sensi- tivity and specificity of experiments showed that the method had high sensitivity and specificity. The mini- mum sensitivity detection limit of LAMP was 10^-5, while the lowest detection limit of Real-time LAMP was 10^-7. All reactions can be completed within 1 h, and the reaction results can be directly judged by the naked eye observation. Initial method which based on the LAMP for rapid detection of common food poisoning bacteria was established, Real-time LAMP results was more sensitive than LAMP.

关 键 词：食源性微生物 LAMP 奇异变形杆菌 Real—time 

分 类 号：R378.3[医药卫生—病原生物学]