两种检测罗氏沼虾诺达病毒方法的比较  

Comparison of Two Detection Methods on Nodavirus of Macrobrachium Rosenbergii

作　　者：简贺君 谢俊刚 

机构地区：[1]肇庆市水产技术推广中心,广东肇庆526060

出　　处：《安徽农学通报》2014年第8期151-152,165,共3页Anhui Agricultural Science Bulletin

摘　　要：分别用三抗体夹心酶联免疫吸附测定、逆转录聚合酶链式反应两种方法,检测20尾采集自广东省肇庆市的典型白肉病症状罗氏沼虾肌肉中的诺达病毒。检测结果是两种方法阳性检出率均为95%。结果表明,两种方法均能有效的应用在实验室检测罗氏沼虾诺达病毒。Three antibodies sandwich ELISA,reverse transcriptase polymerase chain reaction were used re-spectively to detect the Nodavirus from the muscle of 20 Macrobrachium Rosenbergii collected from Zhaoq-ing City,Guangdong Province,which have typical symptoms of white meat disease. The positive rate tested from these two methods was the same： 95%. The results of the comparison showed that both methods are effective on laboratory detection of Macrobrachium Rosenbergii Nodavirus.

关 键 词：罗氏沼虾 诺达病毒 三抗体夹心酶联免疫吸附测定 逆转录聚合酶链式反应 

分 类 号：S966.1[农业科学—水产养殖]