利巴韦林、病毒灵、黄芪多糖体外抗ALV-J的活性试验  

The Activity Experiment of Ribavirin,Moroxydine and Astragalan Against ALV-J

作　　者：王广文[1] 牛玉娟[1] 胡东方[1] 王彤彤[1] 张振东[1] 吕传卫 屈亚锦[1] 李宁[1] 刘思当[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271000

出　　处：《中国兽药杂志》2014年第5期36-39,共4页Chinese Journal of Veterinary Drug

基　　金：公益性行业(农业)科研专项(201203055)

摘　　要：为寻求抗ALV—J感染雏鸡用药物，通过CCK-8试剂盒检测细胞活力以确定利巴韦林、病毒灵和黄芪多糖对DFl细胞的最大安全浓度，用ELISA试剂盒检测ALV—JP27抗原表达量，以确定上述抗病毒药物体外抗ALV—J活性。结果显示，利巴韦林、病毒灵和黄芪多糖对DFl细胞的最大安全浓度分别为0．0391、0．0625和0．0625mg／mL。在安全范围内，利巴韦林对P27的表达量具有明显的抑制作用，黄芪多糖在0．0039～O．0625mg／mL安全浓度范围内明显抑制P27的表达，病毒灵在安全范围内对P27的表达基本上没有抑制作用。试验表明，利巴韦林和黄芪多糖对ALV-J的增殖有明显的抑制作用，有望作为该病净化的辅助性防制药物。To acquire the anti-ALV-J drug, P27 expression quantity influence of Ribavirin, moroxydine and astragalan on ALV-J （NX0101） are researched by the CCK 8 kits cell detected for the maximum safe concentrations of the three antiviral drugs on DF1 cell and ALV-J P27 antigen ELISA test kit for P27 expression quantity.The results show that the maximum safe concentrations of Ribavirin, moroxydine and astragalan on DF1 cell are 0.039, 0.0625 and 0.0625 mg/mL. The obvious inhibitory concentration of Ribavirin for the P27 expression quantity in the the scale of safe concentrations is 0.0391 mg/mL. The concentration range of astragalan inhibiting the expression of ALV-J P27 are 0.0039 - 0.0625 mg/mL. However, varied concentration of moroxydine in the research has no inhabitory effect on P27 expression quantity. The aforementioned results show that ribavirin and astragalan can inhabit the propogation of ALV-J, providing a perfect prospect for the ALV-J eradication in chinken in the future.

关 键 词：利巴韦林 病毒灵 黄芪多糖 ALV—J P27 

分 类 号：S859.79[农业科学—临床兽医学]