黑松—黄色须腹菌共生体根际菌根辅助细菌的筛选及鉴定  

Isolating and Identifying Mycorrhiza Helper Bacteria from the Rhizosphere Soil of Pinus thunbergi Inoculated with Rhizipogen luteous

作　　者：盛江梅[1] 吴小芹[1] 侯亮亮[1] 

机构地区：[1]南京林业大学,南京210037

出　　处：《东北林业大学学报》2014年第5期110-114,共5页Journal of Northeast Forestry University

基　　金：国家林业公益性行业科研专项(201004061);国家"十二五"科技支撑计划专题(2012BAD19B0703);国家自然科学基金项目(30571471);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：采用干皿对抗法和双接种盆栽试验,从黑松-黄色须腹菌(Rhizopogen luteous,Rl)菌根根际土壤中分离筛选获得2株菌根辅助细菌HR10和HR15菌株。离体互作结果表明,菌株HR10和HR15的菌体及胞外代谢产物均可有效提高Rl菌丝的生长,其菌体对Rl菌丝生长的增长率分别达到13.1%和33.6%。盆栽试验表明,HR10和HR15菌株分别与Rl双接种黑松幼苗后对松苗生长有显著的促进作用,同时还有效提高了根系的菌根侵染率,单接Rl处理的菌根侵染率为40.83%,Rl+HR10和Rl+HR15处理的分别为71.54%和60.97%。单接种HR10和HR15也可促进黑松生长,其中单接种HR10处理松苗长势好于双接种。这说明HR10和HR15既是黑松-黄色须腹菌的菌根辅助细菌,也是植物促生细菌,对黑松的生长具有直接的促进作用。经形态学观察、Biolog微生物系统鉴定及16S rRNA分析,菌株HR10和HR15分别被鉴定为短小芽孢杆菌(Bacillus pumilus)和藤黄微球菌(Micrococcus luteus),两者在GenBank数据库中的登录号分别为FJ040808和FJ040810。We isolated two MHB, HR10 and HR15, from the mycorrhizosphere of Pinus thunbergii inoculated with Rhizipogen luteous （R1） by dry-plate confrontation assay and double pot experiment. The confrontation assay shows that HR10 and HR15 can significantly promote the hypbal growth of R1 by 13.1% and 33.6%, respectively. HR10 and HR15 can significantly stimulate P. thunbergii-R, luteous symbiosis. R1 can significantly stimulate the mycorrhizal formation in the presence of HR10 and HR15 （71.54% and 60.97% , respectively）, compared with the control Rl （40.83%）. By the morphological observation, Biolog analysis and 16S rRNA sequencing methods, HR10 and HR15 are identified as Bacillus pumlius and Micrococcus luteus, with the accession number of FJ040808 and FJ040810 in Genbank database, respectively

关 键 词：黄色须腹菌 黑松 菌根辅助细菌 短小芽孢杆菌 藤黄微球菌 

分 类 号：S718.83[农业科学—林学]