非职业多环芳烃暴露人群尿1-羟基芘与外周血TH1和TH2型细胞因子的相关性研究  

Relationship between 1-hydroxypyrene in urine and TH1/TH2 type cytokines in peripheral blood among population non-occupationally exposed to PAHs

作　　者：陈靖晗[1] 王芳[1] 侍洪斌[2] 吴丽娜[1] 吕严[1] 

机构地区：[1]天津医科大学公共卫生学院劳动卫生与环境卫生学系,天津300070 [2]天津医科大学总医院

出　　处：《环境与健康杂志》2014年第3期253-255,共3页Journal of Environment and Health

摘　　要：目的研究健康非职业人群多环芳烃（PAHs）暴露与TH1和TH2型细胞因子表达水平的关系。方法于2012年5月在天津市某医院体检中心选择进行体检的67名健康居民以问卷形式调查基本信息、生活方式等情况，以碱水解-高效液相色谱法测定尿中1-羟基芘浓度，以酶联免疫吸附测定法分别测定血浆中THl型细胞因子（IL-2、IFN-γ）和TH2型细胞因子（IL-4、IL-10）的表达。结果本次调查人群尿1-羟基芘浓度范围为0．21～6．70μmol／mol Cr，中位数为1．13μmol／mol Cr；在调整了研究对象的性别、年龄、电磁辐射和职业性化学试剂接触情况后，尿1-羟基芘浓度与TH1／TH2细胞因子的比值无统计学相关关系（P〉0．05），但随尿1-羟基芘浓度的增加，TH1／TH2比值有逐渐下降的趋势。结论本次调查的非职业暴露人群尿1-羟基芘水平偏高，应对其可能的PAHs高暴露因素开展深入研究。Objective To investigate the relationship between 1- hydroxypyrene（1-OHPyr） in urine and the expression of TH1/ TH2 type cytokines in blood among healthy population non-occupationally exposed to PAHs. Methods A total of 67 healthy volunteers were collected from physical examination center of a hospital in Tianjin in May, 2012, basic information ,life styles and so on were collected by questionnaires. The concentrations of 1-OHPyr in urine samples were determined by alkaline hydrolysis-HPLC, the expressions of TH1 and TH2 type cytokines in plasma were determined by ELISA. Results The range and median of 1-OHPyr in urine samples were 0.21-6.70 μmol/mol Cr, 1.13 μmol/mol Cr respectively, which didn＇t show statistically correlation with ratio of TH1/TH2 type cytokines （P〉0.05） after adjustment of gender, age, electromagnetic radiation and occupational exposure to chemical regent,but an increasing trend with decreasing ratios of TH1/TH2 type cytokines was also found. Conclusion The investigated population reveales higher level of 1-OHPyr in urine,the potential PAHs exposure should be explored.

关 键 词：多环芳烃 1-羟基芘 TH1和TH2型细胞因子 

分 类 号：R181.3[医药卫生—流行病学]