丙泊酚对大鼠大脑睡眠-觉醒相关核团c-fos蛋白表达的影响  

The effect of propofol on c-fos protein expression of nuclein related to sleep-awakening in rat brain

作　　者：薛兴[1] 范坤[1] 张晓兰[1] 

机构地区：[1]甘肃省妇幼保健院麻醉科,兰州市730050

出　　处：《临床麻醉学杂志》2014年第5期503-506,共4页Journal of Clinical Anesthesiology

摘　　要：目的通过研究丙泊酚对大鼠大脑睡眠-觉醒相关核团c-fos蛋白表达的影响,探讨丙泊酚诱导睡眠的可能机制。方法选择SD雄性大鼠24只,随机分为四组:生理睡眠组(A组)、生理觉醒组(B组)、生理盐水组(C组)、丙泊酚组(D组),采用免疫组化法测定大鼠睡眠-觉醒相关核团cfos蛋白的表达。结果与A组比较,B组和C组腹外侧视前区(VLPO)的c-fos免疫阳性反应神经fos-IR表达明显减少(P<0.01);结节乳头体核区(TMN)、穹窿周核区(PeF)和外侧下丘脑区(LH)的fos-IR表达明显增加(P<0.01)。与B组比较,D组VLPO区fos-IR表达明显增加,TMN、PeF和LH区fos-IR表达明显减少(P<0.01)。与C组比较,D组VLPO区fos-IR表达明显增加(P<0.01);TMN、PeF和LH区fos-IR表达明显减少(P<0.01)。四组黑质致密部(SNC)区均无fos-IR表达。结论丙泊酚可能通过兴奋睡眠相关核团VLPO区GABA能神经元及抑制觉醒相关核团神经元调节睡眠-觉醒。Objective To study the effect of propofol on c-los protein expression of nuclein related to sleep-awakening in rats brain and investigate the possible mechanism of propofol-indueed sleeping. Methods Twenty-four SD rats were randomly divided into 4 groups： spontaneous sleep group （group A）, awaken group （group B）, saline group （group C） and propofol group （group D）. Immunohistoehemical method was used to measure c-los protein expression of nuclein area related to sleep-awakeing in rat brain. Results Compared with group A, fos-IR was decreased in the ventrolateral preoptic nucleus （VLPO） in groups B and C; while it was increased in the tuberomammillary nucleus （TMN）, perifornical nucleus （PeF） and lateral hypothalamic area （LH） （P〈0.01）. Compared with group B, fos-IR was increased in the VLPO in group D （P〈0. 01）, while it was decreased in the TMN, PeF and LH （P〈0. 01）. Compared with group C, fos-IR was increased in the VLPO in group D, while it was decreased in the TMN, PeF and LH （P〈0.01）. There was no expression of fos-IR in SNC area in the four groups. Conclusion Propofol moderates sleep-awakening by exciting the GA- BA neurons in the VLPO region of sleep-related nuclein and inhibiting the neurons in awakening-related nuclein.

关 键 词：睡眠-觉醒 C-FOS蛋白 丙泊酚 

分 类 号：R614[医药卫生—麻醉学]